| EB病毒潜伏膜蛋白1对鼻咽癌细胞增殖及STAT3信号通路的影响 |
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| 引用本文： | 吴民华,陈小毅,陈东. EB病毒潜伏膜蛋白1对鼻咽癌细胞增殖及STAT3信号通路的影响[J]. 解剖学杂志, 2010, 33(1) |
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| 作者姓名： | 吴民华 陈小毅 陈东 |
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| 作者单位： | 广东医学院,组织学与胚胎学教研室,湛江,524023;广东医学院,病理学教研室,湛江,524023 |
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| 基金项目： | 广东省自然科学基金,湛江市科技攻关项目 |
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| 摘 要： | 目的:探讨EB病毒编码的潜伏膜蛋白1(LMP1)对人鼻咽癌细胞增殖及信号转导与转录活化因子3(STAT3)信号通路的影响.方法:体外培养人高分化鼻咽癌细胞系CNE1及转染了LMP1基因质粒的CNE1细胞(CNE1-GL细胞),通过绘制细胞生长曲线及平板克隆形成实验比较转染LMP1基因前后细胞增殖能力的变化;RT-PCR及免疫印迹方法检测STAT3及其下游基因survivin表达的变化.结果:转染LMP1基因后,CNE1细胞生长加快、克隆形成率增加;STAT3活化程度增加,survivin表达增强.结论:LMP1可促进CNE1细胞增殖,其机制可能是通过活化STAT3信号通路,进而促进抗凋亡基因survivin表达.
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| 关 键 词： | 鼻咽肿瘤 细胞增殖 潜伏膜蛋白1 信号转导与转录活化因子3 生存素 人鼻咽癌细胞 |
Effects of EBV latent membrane protein 1 on proliferation and STAT3 signaling pathway of nasopharyngeal carcinoma cells |
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| Wu Minhua,Chen Xiaoyi,Chen Dong. Effects of EBV latent membrane protein 1 on proliferation and STAT3 signaling pathway of nasopharyngeal carcinoma cells[J]. Chinese Journal of Anatomy, 2010, 33(1) |
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| Authors: | Wu Minhua Chen Xiaoyi Chen Dong |
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| Abstract: | Objective: To investigate the role of latent membrane protein 1 (LMP1) in the proliferation and STAT3 signaling pathway of nasopharyngeal carcinoma (NPC) cell line CNE1. Methods: CNE1 cells and CNE1-GL cells (CNE1 cells transfected with an eukaryotic LMP1-expression plasmid) were cultured in vitro. Cell growth curves and clone-formation assay were performed to measure the cell proliferation. The expressions of STAT3 and survivin were measured by RT-PCR and Western blot. Results: Transfection of LMP1 gene promoted the proliferation and activation of STAT3 in CNE1 cells, and up-regulated clony forming and expression of phoshated STAT3 (p-STAT3) and survivin. Conclusion: LMP1 may promote the proliferation of CNE1 cells by activating STAT3 signaling pathway. |
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| Keywords: | nasopharyngeal neoplasm cell proliferation latent membrane protein 1 signal transducer and activator of transcription 3 survivin human nasopharyngeal carcinoma cell |
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