| 蛲虫核糖体DNA ITS1的PCR扩增及序列分析 |
| |
| 引用本文： | 邓莉,吴亚敏,彭礼飞,王唯唯. 蛲虫核糖体DNA ITS1的PCR扩增及序列分析[J]. 中国热带医学, 2006, 6(8): 1336-1337 |
| |
| 作者姓名： | 邓莉 吴亚敏 彭礼飞 王唯唯 |
| |
| 作者单位： | 广东医学院寄生虫学教研室,广东,湛江,524023 |
| |
| 摘 要： | 目的 扩增人蛲虫（Enterobius vermicularis）核糖体DNA的第一内转录间隔区（ITS1）。方法提取人蛲虫的基因组DNA，PCR扩增rDNA的ITS1及部分5．8S片段，对该片段进行PCR—SSCP分析并测序。结果扩增的片段大小为334bp，包含Enterobius vermicularis全部的ITS-1（303bp）及部分5．8S（31bp）序列，ITS1种内序列一致。结论首次报道人蛲虫的ITS1序列，将为以ITS1作为遗传标记用于蛲虫鉴别诊断、系统发育等研究奠定基础。
|
| 关 键 词： | 蛲虫 ITS1 PCR—SSCP 序列分析 |
| 文章编号： | 1009-9727（2006）08-1336-02 |
| 收稿时间： | 2006-05-10 |
| 修稿时间： | 2006-05-10 |
PCR amplication and sequence analysis of ITS1 rDNA of Enterobius vermicularis |
| |
| DENG Li, WU Ya - min, PENG Li - fei,et al. PCR amplication and sequence analysis of ITS1 rDNA of Enterobius vermicularis[J]. China Tropical Medicine, 2006, 6(8): 1336-1337 |
| |
| Authors: | DENG Li WU Ya - min PENG Li - fei et al |
| |
| Abstract: | Objective To obtain the sequences of ITS1 of Enterobius vermicularis. Methods The internal transcribed spacer 1(ITS1) and partial 5.8S rDNA of E.vermicularis were amplified by PCR and analysed by single-strand conformation polymorphism(SSCP). Results No variation in single-stranded ITS1 profile was detected among samples,and complete ITS1 was 303bp,and partial 5.8S was 31bp. Conclusion It was the first time that the complete sequence of ITS1 rDNA of E.vermicularis was reported.The results of the present study indicate ITS data may be useful for the diagnosis of E.vermicularis infection and the investigation of phylogenetics. |
| |
| Keywords: | Enterobius vermicularis ITS1 PCR - SSCP Sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
