电针保护大鼠急性胃黏膜损伤基本腧穴配伍“胃病方”的筛选

目的:通过对临床治疗胃病最常用的3个腧穴不同组合配伍的效应比较,选出最优的穴位组合。方法:将48只清洁级Wistar大鼠按析因设计随机分为8组,即模型组、足三里组、中脘组、内关组、足三里+中脘组、足三里+内关组、中脘+内关组、足三里+中脘+内关组。采用无水乙醇灌胃法制作急性胃黏膜损伤模型。除模型组外,其它组大鼠在相应穴位施以电针。电针治疗结束1 h后,取大鼠的胃黏膜组织,分别进行胃溃疡指数的计算、组织学观察,并运用透射电镜进行超微结构观察。结果:7个电针组与模型组相比,胃黏膜损伤指数和病理损伤积分显著降低(P<0.05)。足三里+中脘+内关组与其它6个电针组相比,两项指标降低更显著(P<0.05)。超微结构观察见模型组胃黏膜壁细胞、主细胞内线粒体肿胀,嵴排列紊乱、断裂,甚至溶解;各电针组胃黏膜细胞损伤程度减轻,足三里+中脘+内关组更为明显。结论:同时电针"足三里"内关"和"中脘"减轻胃黏膜损伤的作用优于其单穴或双穴使用,因而可作为治疗胃病的基本处方。

Selection of Basic Acupoints for Composing "Gastric-Disorder-Formula" for Electroacupuncture Prevention of Acute Gastric Mucosal Lesion in Rats

Objective To select the best combination of the commonly-used acupoints Zhongwan(CV 12),Neiguan(PC 6),and Zusanli(ST 36)] for treatment of gastric disorders.Methods Forty-eight Wistar rats were randomly divided into 8 groups: model,ST36,CV12,PC6,ST36+PC6,ST36+CV12,CV12+PC6 and ST36+CV12+PC6 by factorial design method.Acute gastric mucosal lesion model was established by intragastric perfusion of anhydrous alcohol.EA(1-3 mA,30 Hz)was applied to the above-mentioned acupoints for 20 min respectively.And after EA,gastric mucosal lesion index(LI),pathological lesion integral score(PLIS) were calculated and mucosal ultramicrostructural changes observed by optical microscope and transmission electron microscope respectively.Results Compared with model group,the gastric mucosal LI and PLIS of ST36,CV12,PC6,ST36+CV12,ST36+PC6,CV12+PC6,ST36+CV12+PC6 groups all decreased significantly(P<0.05).Comparison among 7 EA groups showed that both LI and PLIS of ST36+CV12+PC6 group were significantly lower than those of ST36,CV12,PC6,ST36+CV12,ST36+PC6 and CV12+PC6 groups(P<0.05).No significant differences were found between every two groups of ST36,CV12,PC6,ST36+CV12,ST36+PC6 and CV12+PC6 groups(P>0.05) in both LI and PLIS.Ultrastructural results showed that the injury of gastric mucosal parietal cells and delomorphous cells including mitochondrial swelling,crista arrange disorder and breaking,enlargement of the rough endoplasmic reticulum,ribosome exfoliation,etc.in model group was serious,but lightened significantly in EA groups particularly in ST36+CV12+PC6 group.Conclusion ST36,PC6 and CV12 combination can effectively reduce anhydrous alcohol-induced gastric mucosal lesion in rats,and may be used as a basic formula for treatment of stomach diseases.