| 药用植物莪术的组织培养快速繁殖与植株再生的研究 |
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| 引用本文： | 李春斌,方宏筠,王关林.药用植物莪术的组织培养快速繁殖与植株再生的研究[J].医学教育探索,2000(11):853-856. |
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| 作者姓名： | 李春斌 方宏筠 王关林 |
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| 作者单位： | [1]辽宁省植物生物工程重点实验室,大连 [2]辽宁师范大学生物工程研究所,大连 |
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| 基金项目： | 辽宁省教委资助的科研攻关项目 |
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| 摘 要： | 通过茎尖培养实现了中药莪术试管苗的快速繁殖并从试管苗基部诱导出了愈伤组织。实验表明：以ＭＳ培养基为基本培养基。附加ＺＴ４．０ｍｇ／Ｌ＋ＩＡＡ０．５～１．０ｍｇ／Ｌ适于丛生芽的诱导与增殖；附加２，４－Ｄ１．０ｍｇ／Ｌ＋ＫＴ４．０～６．０ｍｇ／Ｌ适于愈伤组织的诱导；附加２，４－Ｄ１．０～２．０ｍｇ／Ｌ＋ＫＴ０．２～０．５ｍｇ／Ｌ适于愈伤组织的继代培养；附加ＫＴ４．０ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ适于愈伤组织的分化。
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| 关 键 词： | 莪术 组织培养 快速繁殖 愈伤组织再生 |
Studies on Plantlet Regeneration and Propagation of Cur cuma zedoaria |
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| Li Chunbin,Fang Hongjun,Wa ng Guanlin.Studies on Plantlet Regeneration and Propagation of Cur cuma zedoaria[J].Researches in Medical Education,2000(11):853-856. |
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| Authors: | Li Chunbin Fang Hongjun Wa ng Guanlin |
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| Institution: | Dalian 116029 |
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| Abstract: | Aseptic seedling of the traditional Chinese medicinal plant, Curcuma zedoaria (Christm ) Rosc was successfully propagated by means of shoot culture, and calli were induced from the base of aseptic seedling MS medium with 4 0 mg/L ZT and 0 5 ~ 1 0 mg/L IAA was suitable for the induction and proliferation of fascicular gemma MS medium with 1 0 mg/L 2, 4 D and 4 0 ~ 6 0 mg/L KT was suitable for the induction of calli MS medium with 1 0 ~ 2 0 mg/L 2, 4 D and 0 2 ~ 0 5 mg/L KT was suitable for the subculture of calli MS medium with 4 0 mg/L KT and 0 2 mg/L NAA was suitable for the differentiation of calli |
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| Keywords: | Curcuma zedoaria (Christm) Rosc tissue culture rapid propagation callus regeneratio |
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