| 百合的组织培养 |
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| 引用本文： | 罗丽萍 杨柏云 等. 百合的组织培养[J]. 医学教育探索, 2001, 0(7): 640-642 |
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| 作者姓名： | 罗丽萍 杨柏云 等 |
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| 作者单位： | 罗丽萍（南昌大学生命科学与食品工程学院，江西南昌 330047） 杨柏云（南昌大学生命科学与食品工程学院，江西南昌 330047） 章敏华（南昌大学生命科学与食品工程学院，江西南昌 330047） 蔡奇英（南昌大学生命科学与食品工程学院，江西南昌 330047） |
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| 基金项目： | 江西省一级重点研究项目 |
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| 摘 要： | 目的 研究百合组培快繁的最佳途径。方法 在MS培养基上添加不同的激素配比；采用鳞叶不同部位作为外植体；改变培养方式。结果 确定了外植体产生小鳞茎的最佳培养基为MS＋NAA 0．5mg/L GA3 2.0mg/L；鳞叶的下部小块分化率最高，92．5％；小鳞茎叶脱分化形成愈伤组织后再分化可获得多而健壮的次级小鳞茎；次级小鳞茎采用液体振荡培养可快速增重。结论 采用组织培养方式可进行百合的快速繁殖，使百合种球的工厂化生产成为可能。
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| 关 键 词： | 百合 组织培养 愈伤组织 液体振荡培养 |
| 修稿时间： | 2000-08-02 |
Studies on tissue culture of Lilium brownii var. viridulum |
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| LUO Li-ping,YANG Bai-yun,ZHANG Min-hu,CAI Qi-ying. Studies on tissue culture of Lilium brownii var. viridulum[J]. Researches in Medical Education, 2001, 0(7): 640-642 |
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| Authors: | LUO Li-ping YANG Bai-yun ZHANG Min-hu CAI Qi-ying |
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| Abstract: | Object To develop a best approach for the rapid propagation of Lilium brownii F.E. Brown var. viridulum Baker by tissue culture. Methods Different parts of the bulb were tried as the explant and cultured on different culture media with the additon of different portions of various hormones at various cultural conditions. Results The best medium for the culture of explant bulb was MS NAA 0.5 mg/L and GA 3 2.0 mg/L and the highest induction rate was at the lowest part of the scale leaves, which attained 92.5%. The small bud can further differentiate to form secondary small buds. By liquid-quivering culturing, the weight increase can be accelerated. Conclusion Tissue culture of L. brownii var. viridulum can achieve its rapid propagation, resulting in the possibility for its industrial production. |
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| Keywords: | Lilium brownii F.E.Brown var. viridulum Baker tissue culture callus liquid-quivering culture |
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