| 正交试验法优选从豆制品下脚料中提取大豆皂苷的工艺 |
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| 引用本文： | 陈燕军,张毅贞,等.正交试验法优选从豆制品下脚料中提取大豆皂苷的工艺[J].医学教育探索,2001(7):602-604. |
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| 作者姓名： | 陈燕军 张毅贞 |
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| 作者单位： | 陈燕军（广西中医学院，广西南宁 530001）
张毅贞（广西中医学院，广西南宁 530001）
卢汝梅（广西中医学院，广西南宁 530001）
戴航（广西中医学院，广西南宁 530001）
李健（广西中医学院，广西南宁 530001）
迟嘉松（广西中医学院，广西南宁 530001） |
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| 基金项目： | 广西自治区卫生厅1999年青年基金课题 |
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| 摘 要： | 目的 优选从豆制品下脚料中提取大豆皂苷的最佳提取工艺条件。方法 通过正交试验，以紫外分光光度法测定总皂苷含量为指标，对提取工艺进行考察。结果 影响回流提取的主要因素对乙醇浓度；影响渗漉提取的因素依次为乙醇浓度、溶媒量和渗漉速度。结论 最佳提取工艺条件为8倍量60％乙醇，热回流1次2．5h。
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| 关 键 词： | 豆制品下脚料 大豆皂苷 提取工艺 正交设计 紫外分光光度法 |
Optimization of processes for recovery of soyasaponins from bean product waste by orthogonal design |
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| CHEN Yan-jun,ZHANG Yi-zhen,LU Ru-mei,DAI Hang,LI Jian,CHI Jia-song.Optimization of processes for recovery of soyasaponins from bean product waste by orthogonal design[J].Researches in Medical Education,2001(7):602-604. |
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| Authors: | CHEN Yan-jun ZHANG Yi-zhen LU Ru-mei DAI Hang LI Jian CHI Jia-song |
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| Abstract: | Object To optimize the process for the recovery of soyasaponins from bean product waste. Methods Conditions for the recovery were studied by orthogonal design guided by the yield of soya-saponins as determined by UV absorbance. Results The most influential factor in the recovery by refluxing was the concentration of alcohol; while in the recovery by percolation the influential factors were in the order of concentration of ethyl alcohol, amount of alcohol used and the rate of percolation.Conclusion The optimal recovery process was found to be a single reflux with eight times of 60% alcohol for 2.5 h. |
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| Keywords: | bean product waste soyasaponins extraction process orthogonal design ultraviolet spectrophotometry |
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