| HLA-A位点PCR-SSP基因分型和血清学分型的比较 |
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| 引用本文： | 嵇月华 陈仁彪. HLA-A位点PCR-SSP基因分型和血清学分型的比较[J]. 中国免疫学杂志, 1999, 15(2): 80-81 |
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| 作者姓名： | 嵇月华 陈仁彪 |
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| 作者单位： | [1]上海输血研究所上海血液中心 [2]上海第二医科大学生物学教研室和医学免疫学教研室 |
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| 基金项目： | 上海市卫生局青年科研基金 |
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| 摘 要： | 目的：建立优化ＨＬＡ－Ａ位点ＰＣＲ－ＳＰ分型方法。方法：采用普通扩增仪和Ｅｐｐｅｎｄｏｒｆ管对细胞系ＤＮＡ和３７个健康正常人进行基因分型，血清学检测采用标准淋巴细胞毒试验方法。结果：发现引物浓度和浓度比、ＤＮＡ的纯度及酶的选用，是影响ＨＬＡ－Ａ位点ＰＣＲ－ＳＳＰ分型准确性的重要因素。该方法对３７个标本的基因分型结果与血清学结果相符合。结论：ＰＣＲ－ＳＳＰ方法具有简便准确的优点，可以作为ＨＬＡ－Ａ位
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| 关 键 词： | HLA－A PCR－SSP 基因分型 血清学 |
A comparison between PCR SSP genotyping and serotyping for HLA A locus |
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| JI Yue Hua,FENG Zhe,YANG Ying et al. A comparison between PCR SSP genotyping and serotyping for HLA A locus[J]. Chinese Journal of Immunology, 1999, 15(2): 80-81 |
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| Authors: | JI Yue Hua FENG Zhe YANG Ying et al |
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| Affiliation: | JI Yue Hua,FENG Zhe,YANG Ying et al. Shanghai Institute of Blood Transfusion,Shanghai Blood Center,Shanghai 200051 |
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| Abstract: | Objective:To establish experimental method of PCR SSP for HLA A locus genotyping.Methods:Genomic DNA from 37 non related healthy individuals were tested by using ordinary PCR amplifier and Eppendorf tubes.Serological typing was conducted using standard microcytotoxicity method.Results:It was found that the concentrations and ratios of primers,the purity of DNA sample and the Taq polymerase employed seemed to be crucial for the accuracy of the HLA A locus PCR SSP.The genotyping results obtained with this method for 37 Hans coincide exactly with the serological results.Conclusion:The PCR SSP method for the HLA A locus is apparently a good alternative to the serological typing and could be spread for HLA A typing. |
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| Keywords: | HLA A PCR SSP Genotyping |
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