葡萄多酚对雨蛙肽诱导的急性胰腺炎胰腺组织的保护作用

 目的:研究葡萄多酚对雨蛙肽诱导的小鼠急性胰腺炎（AP）的保护作用。方法: 2月龄ICR雌鼠共21只，随机分为正常对照组（NC组）、AP模型组（AP组）和药物处理AP组，每组7只。造模前，药物处理AP组小鼠连续7 d胃内灌注葡萄多酚（1.5 g/kg）水溶液，NC组和AP组小鼠则以同法给予生理盐水作为对照。第7天开始造模。AP组以及药物处理AP组小鼠予以腹腔注射雨蛙肽(50 μg/kg)造模，每小时1次，共注射7次；NC组小鼠同法注射等量生理盐水。于24 h处死小鼠取其胰腺及肺组织。测量各组胰腺组织的相对重量、病理形态学改变、巨噬细胞的浸润情况及炎症因子、氧化应激分子的表达水平；检测肺脏组织的髓过氧化物酶（MPO）活性以考察远隔器官的炎症。 结果: 与AP组小鼠相比，药物处理AP组小鼠胰腺组织的水肿、炎症以及空泡的评分明显降低（P<0.05），但坏死和病理总评分没有明显变化；巨噬细胞的浸润明显减少；肿瘤坏死因子α(TNF-α)和单核细胞趋化蛋白1(MCP-1)的表达水平也明显降低(P<0.05)；超氧化物歧化酶1（SOD-1)、SOD-2和NADPH氧化酶2（NOX-2）的表达水平也明显降低（P<0.05, P<0.05, P<0.01）。此外，药物处理AP组小鼠肺组织的MPO活性显著降低（P<0.01），提示中性粒细胞的浸润减少。结论: 葡萄多酚对雨蛙肽诱导的小鼠急性胰腺炎胰腺组织具有明显保护作用，机制与下调炎症因子和氧化应激分子的表达有关。

Protective effects of grape polyphenol on pancreatic tissues of mice with caerulein-induced acute pancreatitis

AIM:To investigate the effects of grape polyphenol (GP) on caerulein-induced acute pancreatitis (AP) in mice. METHODS:Two-month-old female mice of ICR strains (n=21) were randomly divided into 3 groups: normal control (NC) group, AP group, and GP-treated AP group. Before AP induction, the mice in GP-treated AP group were continuously administrated with 1.5 g/kg GP aqueous solution by gavage for 7 d, while those in NC group and AP group were treated with saline as a vehicle control. On the 7th day, the mice in AP group and GP-treated AP group were intraperitoneally injected with caerulein (50 μg/kg) in 1 h interval for 7 serial injections in total. The mice in NC group were treated with saline according to the same procedure in experimental group. All the mice were sacrificed 24 h after AP induction, and the pancreatic tissues and lung tissues were harvested for further investigation of the pathological changes, macrophages infiltration, myeloperoxidase (MPO) activity and expression of inflammatory and oxidative stress factors. RESULTS:Compared with AP group, the mice in GP-treated AP group showed milder morphological changes and lower pathological scores, including the scores of edema, inflammation and vacuolization (P<0.05), but the necrosis scores and total scores showed no statistical difference between these 2 groups. Besides, the mice in GP-treated AP group had fewer macrophage infiltration, lower lung MPO activity (P<0.01), and lower expression of inflammatory factors, tumor necrosis factor α (TNF-α)and monocyte chemotactic protein 1 (MCP-1) (P<0.05), and oxidative stress factors, superoxide dismutase (SOD)-1, SOD-2 and NADPH oxidase 2 (NOX-2) (P<0.01). CONCLUSION: Grape polyphenol has remarkable protective effect on pancreatic tissues of mice with caerulein-induced acute pancreatitis, and the mechanisms may be related to down-regulation of inflammatory and oxidative stress factors.