糖尿病患者与健康人脂蛋白单糖组成差异研究

 目的:建立适用于检测人脂蛋白单糖组成的液相色谱方法，探讨糖尿病患者与健康人脂蛋白的单糖组成差异。方法:采用柱前衍生液相色谱法检测脂蛋白上的中性和碱性单糖，液相串联质谱法检测唾液酸的含量。结果:健康人和糖尿病患者高密度脂蛋白上的甘露糖（Man）、葡萄糖胺（GlcN）、N-乙酰葡萄糖胺（GlcNAc）、葡萄糖（Glc）、半乳糖（Gal）和N-乙酰神经氨酸（NANA）的含量依次为：(5.88±0.94)、(16.49±4.11)、(1.31±0.33)、（0.87±0.16）、（7.18±1.64）、（2.14±0.12) mmol/（g protein）和(8.68±0.39）、（24.73±5.50）、（1.91±0.54）、（1.23±0.35）、（9.73±2.85）、（3.53±0.27) mmol/（g protein）。健康人和糖尿病患者低密度脂蛋白上的Man、GlcN、GlcNAc、Glc、Gal和NANA的含量依次为：(29.20±3.57）、（50.77±4.72）、（5.28±0.64）、（10.06±1.37）、（28.44±3.96）、（6.86±0.11) mmol/（g protein）和(30.08±3.78）、（38.52±6.38）、（3.79±0.78）、（7.63±1.50）、（20.05±2.63）、（6.45±0.18) mmol/（g protein）；健康人和糖尿病患者极低密度脂蛋白上的Man、GlcN、Glc、Gal和NANA的含量依次为：(91.21±4.12)、(27.05±2.34)、(4 230.95±15.83)、（43.40±3.75）、（2.95±0.24) mmol/（g protein）和（82.40±0.51)、(30.16±0.32)、(4 722.73±93.27)、（34.05±2.81）、(4.42±0.15） mmol/（g protein）。结论: 本文建立的柱前衍生液相色谱法适用于脂蛋白上的单糖组成分析。糖尿病患者脂蛋白的糖基化修饰与健康人相比存在显著差异。

Comparative study of lipoprotein monosaccharide composition between diabetic patients and healthy participants

AIM:To establish a liquid chromatography method for determining the monosaccharide composition of human lipoproteins, and to investigate the differences between diabetic patients and healthy participants. METHODS:Liquid chromatography with pre-column derivatization was used to determine the neutral and basic monosaccharides, and liquid chromatography tandem mass spectrometry was applied to quantify N-acetylneuraminic acid content. RESULTS:The contents of mannose, glucosamine, N-acetylglucosamine, glucose, galactose and N-acetylneuraminic acid in high-density lipoprotein from healthy participants and diabetic patients were (5.88±0.94),(16.49±4.11),(1.31±0.33), (0.87±0.16), (7.18±1.64), (2.14±0.12) mmol/(g protein) and （8.68±0.39), (24.73±5.50), (1.91±0.54), (1.23±0.35), (9.73±2.85), (3.53±0.27） mmol/(g protein）, respectively. The contents of mannose, glucosamine,N-acetylglucosamine, glucose, galactose and N-acetylneuraminic acid in low-density lipoprotein from healthy participants and diabetic patients were (29.20±3.57), (50.77±4.72), (5.28±0.64), (10.06±1.37), （28.44±3.96）,（6.86±0.11) mmol/(g protein) and (30.08±3.78）, （38.52±6.38）, （3.79±0.78）, （7.63±1.50）, （20.05±2.63）, （6.45±0.18) mmol/(g protein), respectively. The contents of mannose, glucosamine, glucose, galactose and N-acetylneuraminic acid in very-low-density lipoprotein from healthy participants and diabetic patients were (91.21±4.12）, （27.05±2.34）, （4 230.95±15.83）, (43.40±3.75), (2.95±0.24) mmol/(g protein) and (82.40±0.51), (30.16±0.32), (4 722.73±93.27), (34.05±2.81), (4.42±0.15) mmol/（g protein）, respectively. CONCLUSION: Liquid chromatography with pre-column derivatization is suitable for the neutral and basic monosaccharide analysis in human lipoproteins, and the glycosylation of lipoproteins in diabetic patients are significantly changed compared with the healthy controls.