高压氧对荷瘤鼠淋巴细胞功能的影响

目的探讨高压氧（HBO）暴露对淋巴细胞功能的影响。方法将Balb／c小鼠随机分成正常对照组、HBO组、肿瘤组、肿瘤HBO组。用接种S-180腹水癌细胞的方法制成免疫功能低下动物模型。经HBO暴露，取血标本观察淋巴细胞一肿瘤细胞花环率、淋巴细胞数、淋巴细胞百分比；用ELISA双抗夹心法测定小鼠血清IgG浓度；用^3H—TdR掺入法测定脾淋巴细胞增殖；用同位素靶细胞释放法观察NK细胞杀伤活性和LAK细胞活性；取脾脏观察小鼠脾指数的变化。结果（1）淋巴细胞一肿瘤细胞花环率肿瘤HBO组明显高于对照组（P〈0．01）。（2）淋巴细胞计数各组之间均无明显变化。（3）淋巴细胞百分比对照组分别比肿瘤HBO组和肿瘤组高（P〈0．01，P〈0．05），HBO组比肿瘤HBO组高（P〈0．01）。（4）IgG测定各组间无统计学差异。（5）淋巴细胞增殖HBO组比对照组低（P〈0．01），肿瘤HBO组比HBO组低（P〈0．01），肿瘤组比HBO组低（P〈0．01）。（6）NK细胞活性肿瘤组比对照组低（P〈0．05），肿瘤HBO组比肿瘤组高（P〈0．05），HBO组比对照组有降低的趋势（P=0．07）。（7）LAK细胞活性肿瘤组比对照组高（P〈0．05）。（8）脾指数各组之问差异无统计学意义（P〉0．05）。结论压力为0．2MPa，氧体积分数为87％的HBO暴露和／或肿瘤接种对免疫系统的作用是多方面的，有的部分增强，有的部分减弱，值得进一步研究。

The effects of hyperbaric oxygen on the lymphocyte function of tumor-bearing mice

Objective To explore the influence of hyperbaric oxygenation (HBO) on the lymphocyte functions of tumor-bearing mice.Methods Tumor-bearing mice models were established by injecting S-180 cells to male Balb/c mice.After HBO exposure,blood samples were taken and lymphocyte-tumor cell rosette rates,lymphocyte count and lymphocyte percentage were observed.The IgG concentration in the blood serum was determined by ELISA method; the proliferation of spleen lymphocytes was measured by 3H-TDR uptake test; the cytotoxicity of nature killer cells and LAK cells was observed by target cell release method and the spleen index was detected.Results The lymphocyte-tumor cell rosette rate in the HBO-tumor group was significantly higher than that of the control group (P<0.01 ) ; no significant difference in lymphocyte counts was shown in different groups; the lymphocyte percentage in the control group was higher than that in the HBO-tumor group and the tumor group (P<0.01,P<0.05 ) ,and that in the HBO group was higher than that in the HBO-tumor group (P<0.01) ; no statistical significance in the IgG concentration was observed in different groups; the proliferation of lymphocytes in HBO group and in tumor group was lower than that in the control group (P<0.01) ,and that in the HBO-tumor group was lower than that in tumor group ( P<0.01) and in HBO group (P<0.01) ; the cytotoxicity of nature killer cells in the control group was higher than that in the tumor group (P<0.05) ; the cytotoxicity of LAK cells in tumor group was higher than that in the control group (P<0.05 ) ; no statistical significance in spleen index was observed in different groups ( P<0.05 ).Conclusions HBO (87% O2,0.2MPa) had various effects on immune system,strengthening some functions and suppressing some functions.