降纤酶对大鼠局灶脑缺血／再灌后caspase—3 mRNA表达和细胞凋亡的影响

目的:通过观察降纤酶对大鼠脑缺血损伤后半胱氨酸蛋白酶-3(caspase-3)的表达和细胞凋亡的影响,研究其对脑保护机制。方法:采用大鼠线栓法栓塞一侧大脑中动脉(MCAO)制作局灶脑缺血/再灌注模型,健康雄性Wistar大鼠144只,分为生理盐水组和降纤酶两组,用原位杂交和原位末端标记(TUNEL)方法,观察缺血0、1、3、6和24 h;缺血3 h再灌注3、6和24 h;缺血 6 h再灌注3、6和 24 h的caspase-3 mRNA的表达和 TUNEL染色阳性细胞数。结果:降纤酶保护组caspase-3 mRNA的表达和TUNEL染色阳性细胞数明显少于生理盐水组。结论:降纤酶可抑制大鼠脑缺血/再灌注后caspase-3 mRNA的表达和细胞凋亡。

Effects of Defibrase on caspase-3 mRNA Expression and Neuronal Apoptosis in Focal Cerebral Ischemia-reperfusion in Rats

Aim: To investigate the effects of defibrase on caspase-3 mRNA expression and neuronal apoptosis in the focal cerebral ischemia-reperfusion in rats and to discuss its possible protective mechanisms. Methods: 144 healthy male Wistar rats were randomly divided into defibrase group and saline group. Middle cerebral artery occlusion (MCAO) with intraluminal filament occlusion was performed. In situ hybridization and TUNEL were applied respectively to detect caspase-3 mRNA and TUNEL positive neurons. Data of Oh, lh,3h,6h and 24 h following MCAO, t 3 h, 6 h and 24 h reperfusion following 3 h and 6 h MCAO were observed. Results: The expression of caspase-3 mRNA and the TUNEL positive neurons of defibrase group were less than those of sahne group. Conclusion: Defibrase has protective effects on cerebral ischemia-reperfusion in rats. Inhibition of the expression of caspase-3 mRNA and neuronal apoptosis may be involved in the protective mechanism.