| 重组人内皮抑素的表达纯化及对内皮细胞增殖的影响 |
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| 引用本文： | 杨健,杨杰. 重组人内皮抑素的表达纯化及对内皮细胞增殖的影响[J]. 解放军预防医学杂志, 2005, 23(4): 256-258 |
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| 作者姓名： | 杨健 杨杰 |
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| 作者单位： | 广州军区广州总医院科训科,广州,510010;吉林大学公共卫生学院 |
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| 摘 要： | 目的探讨融合表达的重组人内皮抑素对人脐带静脉血管内皮细胞（ECV304）增殖的影响。方法将pTRX—endo质粒转化大肠杆菌BL21（DE3），IPTG诱导表达，经纯化后加入ECV304细胞培养，MTT法检测对内皮细胞增殖的影响。结果经SDS—PAGE分析，出现特异性蛋白质条带，纯化后得到纯度在95％以上的重组人内皮抑素蛋白，明显抑制ECV304的增殖。结论内皮抑素可明显抑制内皮细胞增殖，存在剂量效应关系，本实验为探索内皮抑素抑制血管生成的机制及应用研究打下基础。
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| 关 键 词： | 内皮抑素 内皮细胞 细胞增殖 |
| 文章编号： | 1001-5248(2005)04-0256-03 |
| 收稿时间： | 2005-03-18 |
| 修稿时间： | 2005-03-18 |
EXPRESSION AND PURIFICATION OF RECOMBINANT HUMAN ENDOSTATIN AND ITS EFFECT ON ENDOTHELIAL CELL PROLIFERATION |
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| YANG Jian,YANG Jie. EXPRESSION AND PURIFICATION OF RECOMBINANT HUMAN ENDOSTATIN AND ITS EFFECT ON ENDOTHELIAL CELL PROLIFERATION[J]. Journal of Preventive Medicine of Chinese People's Liberation Army, 2005, 23(4): 256-258 |
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| Authors: | YANG Jian YANG Jie |
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| Abstract: | Objective To investigate the effect of recombinant human endostatin expressed in E.coli on the proliferation of ECV304 cells.Methods pTRX-endo plasmid was expressed in E.coli BL21(DE3).Endostatin was expressed in E.coli by IPTG inducement, ECV304 cell were added to it for culture after purification, and its prolification effect was analyzed by MTT.Results SDS-PAGE analysis showed a specific protein strip,the purity of recombinant human endostatin was over 95% after purification. Endostatin inhibited obviously the proliferation of ECV304 cells. Conclusion Recombinant human endostatin may antagonize the proliferation of endothelial cell.The experiment lays foundation for the research of antiangiogenesis and application of endostatin. |
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| Keywords: | endostatin endothelial cells cell proliferation |
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