Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis |
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| Authors: | Cui Xiao-Nan Tang Jian-Wu Hou Li Song Bo Li Li Liu Ji-Wei |
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| Affiliation: | 1. Department of Oncology, the 1st Affilitate Hospital of Dalian Medical University, Dalian 116011,Liaoning Province, China
2. Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China |
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| Abstract: | AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis. METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed. RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes. CONCLUSION: SSH is a useful technique to detect differentially expressioned genes and an effective method to clone novel genes. |
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| Keywords: | Suppression subtracted hybridization Liver neoplasm Lymphogenous metastasis |
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