| 牛蒡多糖对K562细胞增殖的抑制及其机制的探讨 |
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| 引用本文： | 孟宇,吕俊,齐世美.牛蒡多糖对K562细胞增殖的抑制及其机制的探讨[J].右江民族医学院学报,2014(1):10-11. |
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| 作者姓名： | 孟宇 吕俊 齐世美 |
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| 作者单位： | 皖南医学院生物化学教研室,安徽芜湖241001 |
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| 基金项目： | 皖南医学院中青年基金项目（编号：WK201215） |
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| 摘 要： | 目的研究牛蒡多糖对K562细胞增殖的抑制作用并初步探索其机制。方法 MTT法检测牛蒡多糖对K562细胞增殖的抑制作用,RT-PCR检测BCL-2mRNA、Bax mRNA的表达。结果牛蒡多糖能明显抑制K562细胞的增殖;BCL-2基因表达下调,Bax的基因表达增多。结论牛蒡多糖对K562细胞增殖有抑制作用,其机制可能与BCL-2基因表达下调,Bax的表达上调有关。
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| 关 键 词： | 牛蒡多糖 K562细胞 BCL-2mRNA Bax mRNA |
Inhibition of K562 cell proliferation by burdock polysaccharide and the related mechanism |
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| Meng Yu,Lu Jun,Qi Shimei.Inhibition of K562 cell proliferation by burdock polysaccharide and the related mechanism[J].Journal of Youjiang Medical College For Nationalities,2014(1):10-11. |
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| Authors: | Meng Yu Lu Jun Qi Shimei |
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| Institution: | (Department of Biochemistry, Wannan Medical College, Wuhu 241001,Anhui, China) |
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| Abstract: | Objective To study the inhibitory effect of burdock polysaccharide on K562 cell proliferation and to study its mechanism. Methods An MTT method was used to detect the inhibition of K562 cell prolif- eration by burdock polysaccharide, and an RT-- PCR method was used to detect the expression of BCL-- 2mRNA and Bax mRNA. Results Burdock polysaccharide could significantly inhibit the proliferation of K562 ceils; the expression of BCL--2 decreased, and the expression of Bax gene increased. Conclusion Bur dock polysaccharide can inhibit the proliferation of K562 ceils, and its mechanism may be related to down--reg ulting the expression of BCL--2 gene and up--regulating the expression of Bax. |
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| Keywords: | Burdock polysaccharide K562 ceils BCL--2mRNA Bax mRNA |
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