青蒿琥酯对人源肝星状细胞LX-2增殖、胶原产生以及KLF6表达的影响

目的：探讨青蒿琥酯（Art）对人源肝星状细胞（HSCs）LX-2增殖的影响以及其抗肝纤维化的作用机制。方法：将肝星状细胞LX-2分为对照组和实验组，对照组为细胞培养液，实验组为不同浓度的青蒿琥酯。应用MTT法检测青蒿琥酯对细胞增殖的影响，比色法检测细胞培养上清中乳酸脱氢酶（LDH）活性，酶消化法测定羟脯氨酸（Hyp）含量，Westeinblotting法测定青蒿琥酯对HSCs内KLF-6蛋白表达的影响。结果：与对照组相比，青蒿琥酯能抑制肝星状细胞LX-2的增殖（P〈0．01），且呈剂量-效应和时间-效应关系；青蒿琥酯各浓度组LDH活性与对照组比较，无显著性差异（P〉0．05）；青蒿琥酯能降低肝星状细胞LX-2中Hyp含量（P〈0．01），且呈剂量-效应关系，并能降低KLF6蛋白的表达（P〈0．01）。结论：青蒿琥酯对人源肝星状细胞LX-2有明显的抑制作用，其作用机制是降低胶原的生成以及KLF6蛋白的表达减少，进而抑制肝纤维化的发生。

Effects of artesunate on cell proliferation,collaging generating and KLF6 expression in hepatic stellate cells

Objective： To explore the effect of artesunate on the proliferation of LX-2 cell and to understand the initiatory anti-fibrogenic mechanisms of artesunate. Methods： Hepatic stellate cells （HSCs） LX-2 were divided into control group and artesunate groups with different concentrations. The inhibition of cell proliferation was determined by MTT colorimetric assay. The content of LDH was determined by using colorimetric method while the content of hydroxyproline （Hyp） was determined by colorimetric method. The Western blotting was used for the detection of the expression of KLF-6 protein in HSCs LX-2. Results： The artesunate had an inhibition effect on cell proliferation （P〈 0.01） with dose and time-effect relationships; the activity of LDH in supernatant had no statistical significance compared with control group （P〉0.05）. The content of Hyp was gradually decreased compared with control group （P〈0.01） with dose-effect relationship. The expression of KLF6 protein was decreased （P〈0.01） in dose-dependent manner. Conclusion： Artesunate could markedly inhibit the proliferation of HSCs LX-2; The mechanism may be associated with suppressing the expression of collagen and thus decreasing the expression of KLF6 protein.