含饱和氢气灌注液对大鼠肝脏冷缺血再灌注肺损伤的影响

目的：评价合饱和氢气乳酸林格灌注液对大鼠肝脏冷缺血再灌注所引起的肺损伤的影响。方法：健康雄性Wistar大鼠24只，随机分为对照组（s组）、普通灌注液组（I／R组）及含饱和氢气灌注液组（H组），每组8只，3组给予相应实验处理。3组大鼠均于再灌注6h后经下腔静脉采血离心获取血清，测定血清中肿瘤坏死因子-α（TNF—α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的浓度。经腹主动脉采血监测动脉血气。取血后处死大鼠获取肺脏，检测肺组织丙二醛（MDA）的含量及超氧化物歧化酶（SOD）的活性，光镜下观察肺组织病理学改变。结果：血清中TNF—α、IL-6、IL-113水平为I／R组〉H组〉S组（P〈0．05）；肺组织中MDA浓度为I／R组〉H组〉S组（P〈0．05）；而肺组织中SOD活性S组〉H组〉I／R组（P〈0．05）。各组动脉血PaO2水平为S组〉H组〉I／R组（P〈0．05），各组中PaC02差异无统计学意义（P〉0．05）。S组肺组织形态结构未见明显异常，I／R、H组均见肺组织损伤，H组损伤较I／R组减轻。结论：含饱和氢气乳酸林格灌注液可以降低炎性反应抑制机体脂质过氧化反应，减轻大鼠肝脏冷缺血再灌注肺损伤，改善肺功能。

Effects of hydrogen-rich perfusion on lung injury by hepatic cold ischemia/reperfusion in rat

Objective： To evaluate the effects of hydrogen-rich Lactate Ringer＇s perfusion solution on lung injury caused by hepatic cold ischemia/repeffusion in rats. Methods： Twenty-four healthy male Wistar rats, were randomly divided into control group （S group）, common perfusion solution group （I/R group）, hydrogen-rich liver perfusion solution group （H group） with 8 in each, before corresponding experimental treatment was given. Six hours after reperfusion, serum was obtained by centrifuging the venous blood to detect the level of tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6） and interleukin-1β （IL-1β）. Abdominal aortic blood was taken to monitor arterial blood gas. Lungs of all rats in three groups were removed after venous blood collection from inferior vena cava to detect the level of malondialdehyde （MDA） and superoxide dismutase （SOD） in lungs. The pathological changes of lung were evaluated by light microscopy. Results： The levels of TNF-α, IL-6 and IL- 1β in serum ranked I/R group 〉 H group 〉 S group （P〈0.05）; And the descending order of MDA concentrations in the lung tissue was FR group〉H group〉S group （P〈0.05）; And for SOD activity of lung tissue , S group〉Group H〉I/R group （P〈0.05）. The levels of arterial blood PaO2 ranked group S〉H group〉I/R group （P〈0.05） with no statistically significant difference between groups of PaCO2 （P〉0.05）. The modality and structure of lung tissue in group S were normal. The injury in groups I/R and H were obvious, and the injury in group H was alleviated compared to group I/R. Conclusion： Hydrogen-rich Lactate Ringer＇s liver perfusion solution can reduce the lung injury caused by hepatic cold ischemia/reperfusion injury.