| TIMP-2基因对人脑胶质瘤U87细胞周期与增殖的影响 |
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| 引用本文： | 周晓平,卢旺盛,沈茜,刘建民,岳志健,洪波,胡小吾,许奕.TIMP-2基因对人脑胶质瘤U87细胞周期与增殖的影响[J].中华神经外科疾病研究杂志,2004,3(5):397-400. |
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| 作者姓名： | 周晓平 卢旺盛 沈茜 刘建民 岳志健 洪波 胡小吾 许奕 |
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| 作者单位： | 1. 第二军医大学长海医院,神经外科,上海,200433
2. 第二军医大学长海医院,实验诊断科,上海,200433 |
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| 摘 要： | 目的探讨金属蛋白酶组织抑制因子(TIMP-2)对人脑胶质瘤细胞的抑制作用,主要是对细胞周期与细胞增殖的作用.方法用含TIMP-2基因的重组腺病毒载体,体外转染人胶质瘤细胞系U87,用Western blot检测目的蛋白的表达.通过细胞生长实验,流式细胞仪分析技术检测U87转染前后细胞增殖、周期与凋亡的变化.结果转染AdTIMP-2病毒后的U87细胞TIMP-2蛋白表达上调,转染后对U87细胞的生长有抑制作用,并出现明显的G0 -G1 期阻滞,但无明显的细胞凋亡峰出现.结论重组腺病毒载体介导的TIMP-2基因在体外对人脑胶质瘤细胞系U87 的生长有抑制作用,可作为胶质瘤治疗的有效工具.
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| 关 键 词： | 胶质瘤 细胞周期 增殖 基因治疗 |
| 文章编号： | 1671-2897(2004)03-397-04 |
| 修稿时间： | 2003年4月7日 |
Effect of recombinant adenovirus AdTIMP-2 on the cell cycle and proliferation of U87 glioma cells |
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| ZHOU Xiaoping ,LU Wangsheng ,SHEN Qian ,LIU Jianmin ,YUE Zhijian ,HONG Bo ,HU Xiaowu ,XU Yi.Effect of recombinant adenovirus AdTIMP-2 on the cell cycle and proliferation of U87 glioma cells[J].Chinese Journal of Neurosurgical Disease Research,2004,3(5):397-400. |
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| Authors: | ZHOU Xiaoping LU Wangsheng SHEN Qian LIU Jianmin YUE Zhijian HONG Bo HU Xiaowu XU Yi |
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| Institution: | ZHOU Xiaoping 1,LU Wangsheng 1,SHEN Qian 2,LIU Jianmin 1,YUE Zhijian 1,HONG Bo 1,HU Xiaowu 1,XU Yi 1 1 Department of Neurosurgery,2 Department of Diagnosis,Changhai Hospital,Second Military Medical University,Shanghai 200433,China |
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| Abstract: | Objective To evaluate the effects of adenovirus mediated in vitro gene transfer of tissue inhibitor of metalloproteinases 2 (TIMP 2) in cell proliferation and cell cycle of malignant glioma cells U87.Methods After infection of U87 human glioma cells with the adenovirus vector in vitro , expression of TIMP 2 was confirmed by Western blot. Growth rate was assayed using methyl thiazolyl tetrazolium(MTT)assay to testify the effect of TIMP 2 on proliferation of glioma cells U87.The change of cell cycle and apoptosis was measured by flow cytometry. Results It had been certified that TIMP 2 was expressed stably in glioma cell lines. AdTIMP 2 could inhibit the cell growth and proliferation effectively. The progression of cell cycle of U87 cells was arrested in G 1 phase, whereas the apoptotic rate of U87 was not affected. Conclusion These results demonstrate that TIMP 2 is a potentially useful treatment of malignant gliomas. |
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| Keywords: | TIMP-2 |
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