小儿肾母细胞瘤nm23基因蛋白表达的研究

目的探讨nm23基因蛋白在小儿肾母细胞瘤中的表达及其与肿瘤临床分期、组织结构型、淋巴结转移关系,为判断预后提供依据。方法应用免疫组织化学技术(S-P法)检测24例小儿肾母细胞瘤中nm23基因蛋白表达情况。结果在24例小儿肾母细胞瘤标本中,I、II期及无转移组nm23基因蛋白表达明显高于III期及转移组。nm23基因蛋白阳性表达与肿瘤转移抑制正相关,与肾母细胞瘤分期负相关(P<0.05)。结论检测小儿肾母细胞瘤中nm23基因蛋白表达水平,对评价肿瘤细胞生物学行为,预测肿瘤进展有重要意义。

Expression of nm23 Gene on Invasion and Metastasis in Wilm''''s Tumor

Objective To explore the relationship between the nm23 gene expresion and the clinical staging, typing of histology, metastasis of Wilm's tumor in children. So we can afford a base for Wilm's tumor's diagnosis and prognosis. Methods The protein expressions of nm23 gene in 24 cases of Wilm's tumor were detected with S-P immunohistochemical method. Results The positive rate of stage I, II and non-metastatic group was higher than that of stage III and metastatic group. There was a negative relationship between positive expression of nm23 and the clinical stage and metastasis rate of lymph node of the tumor (P<0.05). Conclusions Detecting the proteins expressive level of nm23 gene in Wilm's tumor have certain reference value for evaluating the biological behavior and predicting tumor recurrence and progression.