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Structure of a Gelasinospora linear plasmid closely related to the kalilo plasmid of Neurospora intermedia
Authors:W Yuewang  X Yang  A J F Griffiths
Institution:(1) Present address: Department of Botany, University of British Columbia, 6270 University Boulevard, V6T 1Z4 Vancouver, B. C., Canada;(2) Present address: Department of Biomedical Sciences, University of Bradford, BD7 1DP Bradford, West Yorkshire, UK;(3) Department of Molecular Genetics, Ohio State University, 43210 Columbus, OH, USA
Abstract:We have determined the complete nucleotide sequence of a linear mitochondrial plasmid from a natural isolate of a homothallic species ofGelasinospora. The plasmid genome is 8231 by long. It carries terminal inverted repeats of 1137 bp. Extending inwards from the terminal repeats are two long open reading frames coding for putative proteins with similarity to DNA and RNA polymerases. These are separated by a short intergenic region. The plasmid sequence shows remarkable similarity to that of theNeurospora intermedia senescence-plasmid kalilo. Overall the two plasmids have a similar genetic organization and are clearly homologous at the sequence level. The main differences are in the intergenic region and in the terminal repeats.
Keywords:Gelasinospora  Neurospora  Plasmid  Mitochondria
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