首页 | 本学科首页   官方微博 | 高级检索  
     

Detection of Ⅴ,Ⅲ and Ⅰ Type Collagens of Dermal Tissues in Skin Lesions of Patients with Systemic Sclerosis and Its Implication
引用本文:刘彤,张键. Detection of Ⅴ,Ⅲ and Ⅰ Type Collagens of Dermal Tissues in Skin Lesions of Patients with Systemic Sclerosis and Its Implication[J]. 华中科技大学学报(医学英德文版), 2008, 28(5): 599-603. DOI: 10.1007/s11596-008-0525-7
作者姓名:刘彤  张键
作者单位:Department of Dermatology, First Affiliated Hospital, Medical School, Xi 'an Jiaotong University, Xi 'an 710061, China
摘    要:This study investigated the contents and distribution of collagen Ⅴ (Col Ⅴ) in skin lesions of the patients with systemic sclerosis (SSc) and its roles in the pathogenesis. The contents and distribution for α1 chain of collagen type Ⅰ, Ⅲ and V [α1 (Ⅰ), α1 (Ⅲ) and α1 (Ⅴ)] in skin lesions of 36 patients with SSc (9 cases of mild fibrosis, 14 moderate, and 13 severe) were detected by using im- munohistochemical SP method. Six cases of normal skin tissues served as controls. The results showed that there was diffuse distribution for three kinds of collagens in dermis. The deep staining α1 (Ⅰ) and α1 (Ⅲ) masses or bands were seen in reticular layer, while α1 (Ⅴ) was distributed more ho- mogeneously. From control to weak, moderate and severe fibrosis stages, α1 (Ⅰ), α1 (Ⅲ) and α1 (V) showed a gradually increased trend in skin lesions (P〈0.05). α1 (Ⅴ) was obviously elevated in skin lesions at early stage and persisted in whole fibrotic process and risen in greater contents, while α1 (Ⅰ) and α1 (Ⅲ) were to go higher late and were apparently elevated at moderate and late stages. Com- pared with α1 (Ⅰ), α1 (Ⅴ) took leading increase at early stage in skin lesions (P〈0.01), and had more elevated contents than α1 (Ⅲ) at moderate and late stages. The fibrotic changes in dermal reticular layer occurred earlier than those in papillary layer, and the abnormalities of α1 (Ⅴ)/α1 (I) ratio ap- peared before α1 (Ⅲ)/α1 (Ⅰ) ratio. It was concluded that a lot of α1 (Ⅴ) began to deposit in greater contents prior to α1 (Ⅰ) and α1 (Ⅲ) at early stage in SSc and persisted in whole fibrotic process. The changes of α1 (Ⅴ) contents in reticular layer occurred earlier than those in papillary layer, and it sug- gested that the fibrosis in reticular layer appeared earlier.

关 键 词:系统性硬化症  真皮组织  胶原蛋白  早期皮损  正常皮肤组织  损伤早期  纤维化  发病机制

Detection of V, III and I type collagens of dermal tissues in skin lesions of patients with systemic sclerosis and its implication
Tong Liu,Jian Zhang. Detection of V, III and I type collagens of dermal tissues in skin lesions of patients with systemic sclerosis and its implication[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2008, 28(5): 599-603. DOI: 10.1007/s11596-008-0525-7
Authors:Tong Liu  Jian Zhang
Affiliation:Department of Dermatology, First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an 710061, China. tonyl1220@sina.com
Abstract:This study investigated the contents and distribution of collagen V (Col V) in skin lesions of the patients with systemic sclerosis (SSc) and its roles in the pathogenesis. The contents and distribution for alpha1 chain of collagen type I, III and V [alpha1 (I), alpha1 (III) and alpha1 (V)] in skin lesions of 36 patients with SSc (9 cases of mild fibrosis, 14 moderate, and 13 severe) were detected by using immunohistochemical SP method. Six cases of normal skin tissues served as controls. The results showed that there was diffuse distribution for three kinds of collagens in dermis. The deep staining alpha1 (I) and alpha1 (III) masses or bands were seen in reticular layer, while alpha1 (V) was distributed more homogeneously. From control to weak, moderate and severe fibrosis stages, alpha1 (I), alpha1 (III) and alpha1 (V) showed a gradually increased trend in skin lesions (P<0.05). alpha1 (V) was obviously elevated in skin lesions at early stage and persisted in whole fibrotic process and risen in greater contents, while alpha1 (I) and alpha1 (III) were to go higher late and were apparently elevated at moderate and late stages. Compared with alpha1 (I), alpha1 (V) took leading increase at early stage in skin lesions (P<0.01), and had more elevated contents than alpha1 (III) at moderate and late stages. The fibrotic changes in dermal reticular layer occurred earlier than those in papillary layer, and the abnormalities of alpha1 (V)/alpha1 (I) ratio appeared before alpha1 (III)/alpha1 (I) ratio. It was concluded that a lot of alpha1 (V) began to deposit in greater contents prior to alpha1 (I) and alpha1 (III) at early stage in SSc and persisted in whole fibrotic process. The changes of alpha1 (V) contents in reticular layer occurred earlier than those in papillary layer, and it suggested that the fibrosis in reticular layer appeared earlier.
Keywords:sclerosis, systemic  V type collagen  immunohistochemical staining
本文献已被 维普 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号