生存素在瘢痕疙瘩组织中的表达及其意义

目的 探讨生存素在瘢痕疙瘩组织中的表达及在瘢痕疙瘩发病中的作用。方法 采用免疫组化SABC法，检测25例瘢痕疙瘩（病例组）及15例正常皮肤（对照组）组织内生存素的表达情况。结果 25例瘢痕疙瘩组织中生存素表达阳性20例，阳性率80.0%，表达部位主要在成纤维细胞和血管内皮细胞内，正常皮肤组织内均表达阴性，两组比较差异有统计学意义（χ2 ＝ 24.00，P < 0.01）。瘢痕疙瘩临床分级低度、中度、重度生存素表达阳性率分别为57.14%（4/7）、81.81%（9/11）、100%（7/7），差异无统计学意义（P ＝ 0.133）；瘢痕疙瘩复发组与未治疗组生存素表达阳性率分别为90.91%（10/11）、71.43%（10/14），差异亦无统计学意义（P ＝ 0.341）。结论 生存素可能参与瘢痕疙瘩的发病。

Expression of survivin in keloid and its clinical significance

Objective To investigate the expression of survivin in keloid, and its significance in the development of keloid. Methods Tissue samples were obtained from 25 patients with keloid (12 males and 13 females, aging from 4 to 44 years with a disease course of 1-18 years), and 15 normal skin samples obtained from surgical operation served as the controls. Streptavidin-biotin-peroxidase complex (SABC) method was applied to detect the expression of survivin in these samples. Results No expressin of survivin was observed in normal controls, while it was expressed in 80.0% (20/25) of the keloid samples with the predominant distribution in fibroblasts and vascular endothelial cells. The positivity rates of survivin were 57.14% (4/7), 81.81% (9/11) and 100% (7/7) in tissues of low-grade, moderate-grade and high-grade keloid, respectively, with no significant difference among the three groups (P = 0.133 ). Similarly, no signifi- cant difference was observed in the positivity rate of survivin between recurrent patients and untreated patients (90.91% (10/11 ) vs 71.43% (10/14), P = 0.341 ). Conclusion Survivin might play a role in the development of keloid.