| 电针对雌性去势大鼠行为学和下丘脑室旁核c-fos表达的影响 |
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| 引用本文： | 申国明,许浩,胡光明,尹刚,王海颖,刘艳.电针对雌性去势大鼠行为学和下丘脑室旁核c-fos表达的影响[J].中华中医药杂志,2011(9):2062-2064. |
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| 作者姓名： | 申国明 许浩 胡光明 尹刚 王海颖 刘艳 |
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| 作者单位： | 安徽中医学院中西医结合临床学院; |
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| 基金项目： | 国家自然科学基金面上项目(No.81072871); 安徽省教育厅自然科学重点科研项目(No.KJ2008A42ZC); 安徽省自然科学基金(No.090413115)~~ |
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| 摘 要： | 目的:观察电针对雌性切除卵巢(去势)大鼠行为学、血浆雌二醇(E2)及下丘脑室旁核c-fos表达的影响,探讨电针的抗抑郁效应及其机制。方法:SPF级SD雌性成年大鼠40只,随机分为正常对照组、去势组、电针组及美雌醇组。除正常对照组外大鼠均行双侧卵巢切除术。2周后,给予双侧内关、三阴交穴位电针干预处理2周。强迫游泳实验(FST)观察行为学变化;免疫组化观察下丘脑室旁核c-fos表达;放射免疫方法检测血浆E2、FSH及LH变化。结果:大鼠卵巢切除4周后,FST显示不动时间明显增加(P<0.01),挣扎时间减少(P<0.05);血浆中E2水平降低(P<0.05),FSH(P<0.01)和LH(P<0.05)水平明显升高。与去势组比,电针组及美雌醇组不动时间明显减少(P<0.01,P<0.05),挣扎时间增加(P<0.05,P<0.01),血浆E2水平明显升高(P<0.01),FSH(P<0.05,P<0.01)和LH(P<0.01,P<0.05)水平明显降低,室旁核c-fos阳性表达明显增强。结论:去卵巢后,可致大鼠行为学改变,表现为抑郁倾向;电针干预具有明显抗抑郁效应,调整下丘脑轴是其可能机制。
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| 关 键 词： | 去势 强迫游泳 雌激素 下丘脑-垂体轴 |
Effect of electroacupuncture on behavioral and the expression of proto-oncogene c-fos in the hypothalamic paraventricular nucleus in female ovariectomized rats |
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| SHEN Guo-ming,XU Hao,HU Guang-ming,YIN Gang,WANG Hai-ying,LIU Yan.Effect of electroacupuncture on behavioral and the expression of proto-oncogene c-fos in the hypothalamic paraventricular nucleus in female ovariectomized rats[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(9):2062-2064. |
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| Authors: | SHEN Guo-ming XU Hao HU Guang-ming YIN Gang WANG Hai-ying LIU Yan |
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| Institution: | SHEN Guo-ming,XU Hao,HU Guang-ming,YIN Gang,WANG Hai-ying,LIU Yan(College of Integrated Chinese and Western Medicine,Anhui University of TCM,Hefei 230038,China) |
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| Abstract: | Objective: To observe the effects of electroacupuncture(EA) on depression-like behavior,estradiol(E2) and the expression of proto-oncogene c-fos in the hypothalamic paraventricular nucleus(PVN) in ovariectomized(OVX) female rats.Methods: SPF grade SD female rats were ovariectomized.After two weeks recovery,the rats in APU group were treated by EA in bilateral Neiguan and Sanyinjiao for two weeks and the rats in E group were treated by mestranol.Later let all the rats in every group to undergo FST.To measure... |
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| Keywords: | Ovariectomy Forced swimming test Estrogen Hypothalamus-pituitary axis |
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