| 麝香酮对氧化应激损伤人血管内皮细胞凋亡的影响 |
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| 引用本文： | 洪艳丽,蒋凤荣.麝香酮对氧化应激损伤人血管内皮细胞凋亡的影响[J].中华中医药杂志,2011(9):2178-2180. |
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| 作者姓名： | 洪艳丽 蒋凤荣 |
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| 作者单位： | 南京中医药大学第一临床医学院;南京中医药大学基础医学院病理教研室; |
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| 摘 要： | 目的:观察麝香酮对过氧化氢(H2O2)诱导人血管内皮细胞(HUVEC)凋亡的保护效应,并探讨其作用机制。方法:运用H2O2建立血管内皮细胞凋亡模型,继而用低、中、高不同剂量的麝香酮进行干预,并用MTT法检测细胞增殖,Hoechst 33258荧光染色及流式细胞技术检测细胞凋亡,激光共聚焦显微镜测定Ca2+浓度和线粒体膜电位(ΔΨm)的变化。结果:H2O2诱导HUVEC细胞凋亡,中、高剂量的麝香酮对HUVEC细胞增殖均有明显促进作用;H2O2组与正常组相比ΔΨm显著下降,Ca2+浓度明显升高(P<0.01);中、高剂量组与H2O2组相比ΔΨm则显著升高,Ca2+浓度明显降低(P<0.01)。结论:麝香酮可通过稳定线粒体ΔΨm,减轻细胞通透性,减少Ca2+内流,从而抑制H2O2所致的HUVEC细胞凋亡。
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| 关 键 词： | 麝香酮 氧化应激 人血管内皮细胞 细胞凋亡 |
Effects of Muscone on human vascular endothelial cells apoptosis induced by oxidative stress |
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| HONG Yan-li,JIANG Feng-rong.Effects of Muscone on human vascular endothelial cells apoptosis induced by oxidative stress[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(9):2178-2180. |
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| Authors: | HONG Yan-li JIANG Feng-rong |
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| Institution: | HONG Yan-li1,JIANG Feng-rong2(1The First Clinical Medical School,Nanjing University of Chinese Medicine,Nanjing 210046,China,2Pathology Faculty Working Office,School of Basic Medical Sciences,China) |
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| Abstract: | Objective: To explore the protection effects of Muscone on H2O2-induced apoptosis in the human vascular endothelial cells(HUVEC).Methods: Apoptosis model of HUVEC was established by inducing of H2O2.Then cells were treated with Muscone at concentrations of low middle and high,respectively.MTT assay was used to observe the cells'growth.Apoptotic rate of HUVEC was detected by Hoechst 33258 fluorescence staining and flow cytometry.Mitochondrial membrane potential and Ca2+ concentration in HUVEC was determined ... |
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| Keywords: | Muscone Oxidative stress Human vascular endothelial cells(HUVEC) Apoptosis |
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