夏枯草、浙贝提取物对体外培养TAO眼眶成纤维细胞的影响

目的探讨夏枯草、浙贝治疗甲状腺相关眼病（TAO）的机制。方法采用体外培养的正常人和TAO患者的眼眶成纤维细胞，将夏枯草、浙贝提取物、刺激药物干扰素-γ和阳性对照药物地塞米松分成不同质量浓度组，以MTT法、磁性均相化学发光免疫分析法、流式细胞仪检测其对细胞的增生、透明质酸（HA）的分泌以及黏附分子（ICAM-1）表达的影响。结果夏枯草、浙贝提取物具有与地塞米松相似的抑制作用，对于TAO患者HA的分泌，133mg／mL的夏枯草质量浓度组与10ug／mL地塞米松组比较差异无统计学意义（P〉0．05），但浙贝各质量浓度组较夏枯草和地塞米松弱（P〈0．01）；对于ICAM—1的表达，夏枯草组的抑制作用较地塞米松组弱（P〈0．01），但较浙贝组强（P〈0．01）。结论夏枯草、浙贝对TAO的治疗作用可能是通过抑制眼眶成纤维细胞的增生、在IFN-γ刺激下HA的分泌和ICAM-1的表达而实现的，且夏枯草作用强于浙贝。

The influence of prunella and fritillaria thunbergii's extract on cultured orbital fibroblasts of TAO-patients

Objective The aim of this study was to discuss the treatment mechanism of the prunella and fritillaria thunbergii to thyroid-associated ophthalmopathy （TAO） patients. Methods The orbital adipose tissue was obtained from 4 patients with TAO during the surgery and 2 normal subjects underwent enucleated eyesballs due to corneal penetrating injury. Collected orbital adipose tissue was incubated using explant culture to harvest the orbital fibroblasts. Prunella and fritillaria thunbergii extract, IFN-γ and dexamethasone were added into medium respectively. The proliferation of the orbital fibroblasts were detected to evaluate the influence of drugs above by MTT, and the secreting HA function of cultured orbital fibroblasts was assessed by the homogeneous magnetic chemiluminescence method immunizations. The expression of adhesion molecule（ ICAM- 1 ） in the fibroblasts was detected by using flow cytometry. Results The migration and passage time seemed to be similar between orbital fibroblasts from TAO patients and ones from normal subjects. Cultured orbital fibroblasts showed the positive immunostaining for Vimentin and absent staining for Desmin, S-100 and CK. Prunella and fritillaria thunbergii extract showed a similar result to dexamethasone in inhibiting the proliferation of cultured orbital fibroblast in TAO patients. The same effect of prunella and fritillaria thunbergii extract on the secretion HA of orbital fibroblast and expressing ICAM-1 in orbital fibroblasts after IFN-γ stimulation was also seen. In different concentrations of prunella groups,only 133 mg/mL of prunella presented the similar function to dexamethasone in inhibiting HA secretion of orbital fibroblast in TAO patient,and fritillaria thunbergii had the weaker effect in comparison with prunella. In the inhibition of ICAM-1 expressing, the effect of prunella was weaker than that of dexamethasone but better than that of fritillaria thunbergii. Conclusion Prunella and fritillaria thunbergii can treat TAO perhaps by inhibiting the proliferati