Mouse oocytes injected with cryopreserved round spermatids can develop into normal offspring |
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Authors: | Atsuo Ogura Junichiro Matsuda Toshihiko Asano Osamu Suzuki R Yanagimachi |
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Institution: | (1) Department of Veterinary Science, National Institute of Health, Shinjuku, 162 Tokyo, Japan;(2) Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, 96822 Honolulu, Hawaii |
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Abstract: | Purpose: This study was performed to determine whether frozen-thawed mouse round spermatids can fertilize oocytes and contribute to normal embryo development.
Methods: Freshly collected mouse testicular cells were frozen in PBS containing 7.5% glycerol and 7.5% fetal bovine serum. After thawing and removal of the cryoprotectants, round spermatids were selected and injected individually into mature oocytes which had been previously activate with Sr
2+
-containing Ca
2+
-free medium.
Results: After thawing, 75–85% of testicular cells were alive. About 90% of the oocytes were fertilized by intracytoplasmic injection of frozen-thawed round spermatids; 11% (17/150) of embryos transferred to foster mothers developed into normal offspring.
Conclusions: Mouse round spermatids can be cryopreserved for production of normal offspring. |
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Keywords: | cryopreservation embryo transfer microinsemination mouse round spermatid |
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