| 无偿献血人群HCV感染的检测和输血残余风险分析 |
| |
| 引用本文： | 欧阳玲,黄建国,谢秀华,黄守民.无偿献血人群HCV感染的检测和输血残余风险分析[J].江西医学检验,2010(4):344-346. |
| |
| 作者姓名： | 欧阳玲 黄建国 谢秀华 黄守民 |
| |
| 作者单位： | 广东省深圳市宝安区中心血站; |
| |
| 基金项目： | 深圳市宝安区科技计划项目资助(2002B061) |
| |
| 摘 要： | 目的了解深圳地区无偿献血人群HCV感染状况,评估血液经EIA筛查抗-HCV后经血传播HCV感染的残余风险。方法采用两种EIA试剂对献血者血液进行抗-HCV筛查,采用核酸检测技术检测EIA检测＂合格＂标本中HCV RNA,对HCVRNA阳性标本进行荧光定量PCR检测病毒载量,并对PCR扩增产物进行测序和病毒基因亚型分析。结果共筛查1997～2009年期间的献血者254 570人份,发现抗-HCV阳性1401人份,阳性率为0.55%;对2002～2007年期间EIA检测＂合格＂的113639份献血者血液标本进行NAT检测,检测出1份HCV RNA阳性、抗-HCV阴性的血清转换窗口期感染的献血者血液,HCV残余风险高达1/113 639。结论 EIA筛查后血液安全性有了很好的保障,但由于HCV感染窗口期存在,经血传播HCV残余风险依然处于较高的水平,NAT应用对提高血液安全,降低输血传播HCV残余风险意义重大。
|
| 关 键 词： | 献血者 丙型肝炎病毒 核酸扩增检测 残余风险 |
The evaluation of residual risk of transfusion by HCV antibody detection in voluntary blood donors |
| |
| OUYANG Ling,HUANG Jianguo,XIE Xiuhua,et al..The evaluation of residual risk of transfusion by HCV antibody detection in voluntary blood donors[J].Jiangxi Journal of Medical Laboratory Sciences,2010(4):344-346. |
| |
| Authors: | OUYANG Ling HUANG Jianguo XIE Xiuhua |
| |
| Institution: | OUYANG Ling,HUANG Jianguo,XIE Xiuhua,et al.Shenzhen Baoan Blood Center,Guang dong 518101,China |
| |
| Abstract: | Objective To estimate the residual risk of transfusion-transmitted HCV infection in blood do-nations in Shenzhen.Methods The blood samples of donors were tested for HCV antibody by enzyme immunoas-say(EIA).The blood donation of EIA-negative reaction for HCV RNA were tested by MP-NAT.The anti-HCV positive plasmas were further detected for HCV RNA by RT-PCR.The partial genome of HCVE1 and NS5B was sequenced and genotyped.Results Of 254 570 blood donations during 1997-2009,1401 samples with anti-HCV positive w... |
| |
| Keywords: | Blood donors Hepatitis C virus Nucleic acid amplification test Residual risk |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
