内皮素-1及其基因在早期STZ糖尿病大鼠肾组织中的表达及其意义

为探讨内皮素１（ Ｅ Ｔ１）及其基因在早期链脲　菌素 （ Ｓ Ｔ Ｚ） 糖尿病大鼠肾脏组织中的表达及其意义，作者采用免疫组化 Ａ Ｂ Ｃ法和原位杂交技术检测了２４只早期 Ｓ Ｔ Ｚ糖尿病大鼠肾组织中 Ｅ Ｔ１及 Ｅ Ｔ１ ｍ Ｒ Ｎ Ａ 的定位及半定量表达。实验分为４组，每组６只。结果： Ｅ Ｔ１和 Ｅ Ｔ１ ｍ Ｒ Ｎ Ａ 的分布一致，主要分布于肾小球内皮细胞、系膜细胞、血管内皮细胞、平滑肌细胞及肾小管上皮细胞之中。髓质中 Ｅ Ｔ１和 Ｅ Ｔ１ ｍ Ｒ Ｎ Ａ 的表达高于皮质， 在糖尿病发生后１～２周， 二者在肾小球内均明显减少 （ Ｐ＜ ００５）， 但在４周时开始增高 （ Ｐ＜ ００５）， 而且在髓质中持续性增高。上述结果表明， Ｅ Ｔ１及 Ｅ Ｔ１ ｍ Ｒ Ｎ Ａ 参与了早期 Ｓ Ｔ Ｚ糖尿病大鼠肾小球高灌注及有关小管间质损伤。

Expression of ET-1 and Pr ET-1 mRNA in Kidney of Early Diabetic Rats

The purpose of this study was to explore the role of endothelin 1 (ET 1) and pr ET 1 mRNA in kidney of early diabetic rats. The location and level of ET 1 were determined by immuno histochemical staining; ET 1 cDNA probe was used for in situ hybridization. The expressions of ET 1 and ET 1 mENA in the kidneys of 24 early diabetic rats were detected by microscopy and Mia 2000 patho image analysis system. The results revealed that the ET 1 mRNA and ET 1 were principally distributed in the glomerular endothelial cells, mesangial cells, smooth muscle cells of vessels, epithelial cell lining tubules and collecting ducts, showing no difference in the distribution over kidney, but the expressions of ET 1 and ET 1 mRNA were higher in the renal medulla. The levels of ET 1 and ET 1 mRNA in glomeruli significantly decreased at week 1 and week 2( P <0 05),but increased at week 4( P <0 05).However, the levels of ET 1 and ET 1 mRNA continually increased from week 1 to week 4 in the medulla of diabetic rats. These data suggest that ET 1 and ET 1 mRNA may induce the glomerular hyperperfusion and damage the tubulointer stitium of the kidneys in early diabetic rats.