| 靶向趋化因子受体5短肽的筛选及活性初步分析 |
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| 引用本文： | 谢惠芳,谭志军,邓霞林.靶向趋化因子受体5短肽的筛选及活性初步分析[J].右江民族医学院学报,2010,32(3):299-302. |
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| 作者姓名： | 谢惠芳 谭志军 邓霞林 |
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| 作者单位： | 湖南省祁阳县人民医院检验科,湖南,祁阳425100 |
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| 摘 要： | 目的筛选人趋化因子受体5(CCR5)的亲和短肽。方法采用噬菌体十二肽库对稳定表达CCR5的中国仓鼠卵巢(CHO)细胞(CHO/CCR5)进行筛选,得到30个阳性克隆,通过酶联免疫吸附试验(ELISA)分析,发现其中17个克隆含有AFDWTFVPSLIL基序。结果含有该序列的噬菌体和合成肽AFDWTFVPSLIL能阻止单克隆抗体2D7与CHO/CCR5细胞的结合;并能竞争性地抑制RANTES对CHO/CCR5细胞的结合。结论小分子肽AFDWTFVPSLIL能特异性地结合CCR5,并有可能成为CCR5的拮抗剂。
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| 关 键 词： | 肽类 受体 CCR5 |
Screening short peptides affinity to chemokine receptors CCR5 and analyzing their activity |
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| XIE Hui-fang,TAN Zhi-jun,DENG Xia-lin.Screening short peptides affinity to chemokine receptors CCR5 and analyzing their activity[J].Journal of Youjiang Medical College For Nationalities,2010,32(3):299-302. |
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| Authors: | XIE Hui-fang TAN Zhi-jun DENG Xia-lin |
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| Abstract: | Objective To screen the short peptides affinity to chemokine receptors CCR5(CCR5). Methods The peptides that bind to Chinese hamster ovarian(CHO) cells that stably expressed CCR5(CHO/CCR5) were identified from Ph.D.-12 phage display peptide library.30 positive clones were obtained.The ELISA technique was used to analyze the gene sequence.17 of the 30 positive clones contained with AFDWTFVPSLIL gene sequence. Results The phage that contained this gene sequence and synthetic peptide AFDWTFVPSLIL blocked the binding of monoclonal antibody 2D7 to CHO/CCR5 cells,and could competitively inhibit the binding of RANTES to CHO/CCR5 cells. Conclusion Small peptide AFDWTFVPSLIL can specifically bind to CCR5 and might be the antagonist of CCR5. |
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| Keywords: | peptides receptors CCR5 |
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