超声生物显微镜成像对正常小鼠心血管的初步研究

目的探讨超声生物显微镜对小鼠心血管成像的方法及价值。方法选择8、16、24及32周龄C57BL／6健康成年雄性小鼠32只，每组各8只，采用Visualsonics公司Vevo770小动物超声成像系统，经胸骨左缘、心尖、胸骨右缘及胸骨上窝等扫查窗口，观察并分析小鼠心脏、大血管的结构及血流频谱，并对左心收缩及舒张功能进行评价。结果经上述扫查窗口，可获得左心室长轴、左心室短轴、升主动脉长轴、主动脉弓长轴、主动脉短轴、右心室流人道、肺动脉长轴等切面，仅心尖切面显示不满意。通过这些切面能清晰显示左心房、左心室、二尖瓣、主动脉及主动脉弓、室间隔、右心房、右心室、三尖瓣、肺动脉、无名动脉及左颈总动脉等结构，进而测量其内径，并可顺利获得各瓣口的血流频谱。各切面心脏及大血管二维径线测值及各瓣口血流脉冲多普勒测值比较显示，各周龄组相关测量数据间差异无统计学意义（P〉0．05）。结论超声生物显微镜可很好地应用于小鼠心脏及大血管成像，采用适当的扫查窗口和切面可获得满意的图像。超声医师经过短期培训即可熟练掌握该技术，因而具有很好的应用前景。

The study of cardiovascular imaging in healthy mice by using ultrasound biomicroscopy

Objective The aim of this study was to explore the methodology of ultrasound biomicroscopy(UBM)in mice cardiovascular imaging and to evaluate the feasibility and value of UBM in mice cardiovascular imaging.Methods Thirty-two C57BL/6 adult male mice were chosen and the Visualsonics Vevo 770 UBM was used in this study.The structure and flow of mice heart and great vessels were shown and analyzed through several acoustic windows by UBM including left parasternal,right parasternal,apical regions,and fossa supra...