| 蛋白激酶C调节脊髓神经元突起的生长(英文) |
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| 引用本文： | 杨萍,李振强,宋林,殷玉芹. 蛋白激酶C调节脊髓神经元突起的生长(英文)[J]. 中国神经科学杂志, 2010, 0(2) |
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| 作者姓名： | 杨萍 李振强 宋林 殷玉芹 |
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| 作者单位： | 第三军医大学解剖学教研室;第三军医大学神经生物学教研室;哈佛医学院儿童医院神经外科;神经科学实验室; |
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| 基金项目： | supported by the National Natural Science Foundation of China (No. 39570373) |
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| 摘 要： | 目的关于蛋白激酶C(PKC)在神经元突起生长和神经再生中的作用,目前仍存有争议。本研究主要观察PKC对离体培养的脊髓神经元生长的调节作用,旨在阐明PKC对突起生长的调节作用。方法分离纯化胎龄14天(E14)的SD胎鼠的脊髓前角神经元,进行原代培养,并检测不同时相点膜/浆PKC活性(m/c-PKCactivity)的比值。结果神经元培养3-11d期间,神经元内m/c-PKC比值以及PKC-βII在突起中的表达水平均与突起生长呈显著相关关系(r=0.95,P<0.01;r=0.73,P<0.01)。此外,PKC激动剂PMA能显著提高m/c-PKC比值,且与神经突起的生长一致(r=0.99,P<0.01)。而PKC抑制剂GF109203X则能显著抑制突起生长,且不被PMA作用所逆转。结论PKC的活性在脊髓神经元突起生长调节中具有重要作用,其中βII亚型可能扮演重要角色。
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| 关 键 词： | 蛋白激酶C 脊髓神经元 突起生长 离体 |
Protein kinase C regulates neurite outgrowth in spinal cord neurons |
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| Ping YANG,,Zhen-Qiang LI,Lin SONG,Yu-Qin YIN. Protein kinase C regulates neurite outgrowth in spinal cord neurons[J]. Neuroscience Bulletin, 2010, 0(2) |
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| Authors: | Ping YANG Zhen-Qiang LI Lin SONG Yu-Qin YIN |
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| Affiliation: | Ping YANG1,2,Zhen-Qiang LI1,Lin SONG1,Yu-Qin YIN3 1Department of Anatomy,Third Military Medical University,Chongqing 400038,China 2Department of Neurobiology,China 3Laboratory for Neuroscience,Department of Neurosurgery,Children's Hospital,Harvard Medical School,Boston 02115 |
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| Abstract: | Objective The functional roles of protein kinase C(PKC) in the neurite outgrowth and nerve regeneration remain controversial.The present study was aimed to investigate the role of PKC in neurite outgrowth,by studying their regulatory effects on neurite elongation in spinal cord neurons in vitro.Methods The anterior-horn neurons of spinal cord from embryonic day 14(E14) Sprague-Dawley(SD) rats were dissociated,purified and cultured in the serum-containing medium.The ratio of membrane-PKC(mPKC) activity to cy... |
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| Keywords: | protein kinase C spinal cord neurons neurite outgrowth in vitro |
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