| 二步温控法聚合酶链反应检测宫颈癌中HPV-16E_6/E_7相关序列 |
| |
| 引用本文： | 刘学锋,杨平,赵文先,丁华,伍欣星.二步温控法聚合酶链反应检测宫颈癌中HPV-16E_6/E_7相关序列[J].武汉大学学报(医学版),1992(2). |
| |
| 作者姓名： | 刘学锋 杨平 赵文先 丁华 伍欣星 |
| |
| 作者单位： | 湖北医学院生化教研究室，湖北医学院病毒所分子生物学室，湖北医学院病毒所分子生物学室，湖北医学院病毒所分子生物学室，湖北医学院病毒所分子生物学室 |
| |
| 基金项目： | 国家“七五”科技攻关专题基金资助 |
| |
| 摘 要： | 应用改良的二步温控法 PCR 技术,检测34例人宫颈癌组织 DNA,发现宫颈癌中 HPV-16 E_6/E_7相关序列存在的阳性率为67.6%(23/34)。结果提示:HPV-16可能在宫颈癌的发生发展过程中起重要作用,采用改良 PCR 技术选择性地检测 HPV-16的转化基因(E6、E7)对深入探讨宫颈癌发生的机理、早期发现宫颈癌患者等有重要意义。
|
| 关 键 词： | 乳头状瘤病毒 子宫颈肿瘤 |
Use of Two-protocol Polymerase Chain Reaction for Detection of the HPV-16 E_6/E_7-related Sequences in Chinese Cervical Cancers |
| |
| Liu Xuefeng Yang Ping Zhao Wenxian Ding Xiaohua Wu Xingxin.Use of Two-protocol Polymerase Chain Reaction for Detection of the HPV-16 E_6/E_7-related Sequences in Chinese Cervical Cancers[J].Medical Journal of Wuhan University,1992(2). |
| |
| Authors: | Liu Xuefeng Yang Ping Zhao Wenxian Ding Xiaohua Wu Xingxin |
| |
| Institution: | Liu Xuefeng Yang Ping Zhao Wenxian Ding Xiaohua Wu Xingxin Department of Molecular Biology,Virus Research Institute,Hubei Medical College,Wuhan 430071,China |
| |
| Abstract: | The modified two-protocol PCR technique was used for detection of the HPV-16 E_6/E_7-related sequences in 34 Chinese cervical cancers,the positive percentage of HPV-16 E_6/E_7 in cervical cancers, was 67.6%(23/34).This result indicated that HPV-16 might play an important role in the develop- ment and progression of cervical cancers,and use of the modified PCR technique for selective detection the transforming genes(E_6/E_7)of HPV-16 may be helpful with research of cervical carcinogegesis and early finding of cervical cancer patients. |
| |
| Keywords: | Mesh papillomavirus cervix neoplasms |
| 本文献已被 CNKI 等数据库收录！ |
