| 唾液富脯蛋白的分离提纯 |
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| 引用本文： | 杨萍 武栋成. 唾液富脯蛋白的分离提纯[J]. 牙体牙髓牙周病学杂志, 1995, 5(3): 131-133 |
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| 作者姓名： | 杨萍 武栋成 |
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| 作者单位： | 武汉市湖北医科大学口腔医学院，武汉市湖北医科大学生化教研室，深圳市龙岗区第二人民医院口腔科 |
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| 摘 要： | 收集刺激性腮腺唾液１０００ｍｌ，利用先进的生化技术分离提纯出唾液富腈蛋白（ＰＲＰｓ）。经硫酸铵浓度差超速离心后得到初步纯化的ＰＲＰｓ，再经葡聚糖凝胶（Ｓｅｐｈａｄｅｘ）Ｇ－７５过滤和ＤＥＡＥ Ｓｅｐｈａｄｅｘ Ａ－２５离子交换层析后可得到ＰＲＰｓ的６个组份。其分子量大小分别为１４３００、１０８００、１１０００、１２３５０、１３０００和１２５８０ｕ；等电点范围在４．１８￣４．６０之间。６种组分的ＰＲ
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| 关 键 词： | 唾液高腈蛋白 分离 提纯 |
Isolation and purification of proline-rich proteins from human parotid saliva |
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| Yang Ping,Yue Jinqiu,Wu Dongcheng.. Isolation and purification of proline-rich proteins from human parotid saliva[J]. Chinese Journal of Conservative Dentistry, 1995, 5(3): 131-133 |
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| Authors: | Yang Ping Yue Jinqiu Wu Dongcheng. |
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| Abstract: | In this study,we collected stimulatcd parotid saliva(1000ml),purified the PRPs from parotid saliva by some advanced biochemical technicalities.Crude PRPs could be obtained through(NH4)2SO4 ultra-centrifugation.Six purified PRPs were isolatcd after Gel filtration on a sephadex G-75 column and ion-change chromatography on a DEAE sephadex A-25 column.The molecular weight for PRPs were 14300,10800,11000,12350,13000 and 12580u respectively.The range of Amino acid composition showed that they all had a large amount of proline,glycine and glutamic acid. |
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| Keywords: | Proline-rich proteins(PRPs) Gel filtration Ion-change chromatography Amino acid composition. |
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