多肿瘤抑制基因p16、RB与喉癌组织细胞增生活性的关系

目的：研究PCNA免疫染色和AgNOR计数在鉴别喉部良恶性病变和判断喉癌的生物学行为中的意义，及其与p16、RB基因表达的关系。方法：采用PCNA LI和AgNOR计数判定喉癌细胞增殖活性，评价喉癌的生物学行为，利用免疫组化方法分析54例喉癌组织中p16、pRb蛋白的表达在肿瘤恶性进展及转化中的作用，及其相互关系。结果：随肿瘤恶性程度的增高，其组织细胞增殖活性越强。p16的抑癌作用有赖于RB的存在，在RB存在的情况下p16可以抑制肿瘤细胞的增殖。结论：与p16、RB、cyclinD1／CDK4、6及转录因子在内的调节通路与细胞增殖周期有关。p16、RB正是通过这一通路对细胞增殖周期进行调节。

Relationship between Expression of Multiple Tumor Suppresser Gene-p16,RB and Proliferative Activity of Laryngeal Carcinoma Tissues

Objective:To study the relationship between expression of multiple tumor suppresser gene-p16,RB and proliferative activity of laryngeal carcinoma tissues.Methods:The proliferative activity of laryngeal carcinoma tissues was evaluated with PCNALI and AgNOR.Results:PCNALI and the average number of AgNOR were correlated with the histopathological grade and clinical stage of laryngeal carcinomas.So the malignant degree had significantly positive relation with proliferative activity.The RB gene could inhibit the growth of carcinoma.The inhibiting function of p16 relied on the existence of RB gene.Conclusion:There is a feedback regulatory loop involving p16,RB and cyclin-dependent kinases.