鼻咽癌细胞凋亡与血管生成的关系

目的：探讨鼻咽癌（ｎａｓｏｐｈａｒｙｎｇｅａｌ ｃａｒｃｉｎｏｍａ，ＮＰＣ）细胞凋亡及瘤内血管生成与患者预后的关系。方法：采用ＴｄＴ酶介导的生物素化ｄＵＴＰ缺口末端标记技术（ＴＵＮＥＬ）和免疫组化Ｓ＝Ｐ法，分别检测２０例正常鼻咽粘膜及７３例ＮＰＣ组织的细胞凋亡率（ａｐｏｐｔｏｓｉｓ ｒａｔｅ，ＡＲ），瘤内微血管密度（ｍｉｃｒｏｖｅｓｓｅｌ ｄｅｎｓｉｔｙ，ＭＶＤ）及血管内皮细胞生长因子（ｖａｓｃｕ

Relationship between apoptosis and angiogenesis in nasopharyngeal carcinoma

Objective:To investigate the relationship among apoptosis, intratumoral microvessel density (MVD) and prognosis of nasopharyngeal carcinoma (NPC) patient Methods:Using terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL) and immunohistochemistry S P method, the apoptotic rate (AR) (number of apoptotic cells/total number of tumor cells), MVD and VEGF expression were examined in normal nasopharyngeal mucosa (20 cases) and NPC tissues (73 cases including 24 recurrent metastatic cases and 49 nonrecurrent cases) Results:Few apoptotic cells were found in NPC The AR of normal tissues was significantly higher than that of NPC(P< 0 01) MVD and VEGF of recurrent nasopharyngeal carcinoma (R NPC) were higher than that of nonrecurrent nasopharyngeal carcinoma (NR NPC) (P< 0 05) , while AR was lower in R NPC than in NR NPC Expression of VEGF significantly correlated with MVD, and AR with VEGF (P< 0 05) Conclusions:Apoptosis is inhibited during the process of nasopharyngeal carcinogenesis VEGF is involved in the angiogenesis of NPC, and angiogenesis influences the prognosis of the patient Angiogenesis and apoptosis may be useful to predict the prognosis of NPC patients