Axonal sprouting of a brainstem-spinal pathway after estrogen administration in the adult female rhesus monkey

The nucleus retroambiguus (NRA) is located in the caudal medulla oblongata and contains premotor neurons that project to motoneuronal cell groups in the brainstem and spinal cord. NRA projections to the lumbosacral cord are species specific and might be involved in mating behavior. In the female cat, this behavior is estrogen dependent, and estrogen induces axonal sprouting in the NRA-lumbosacral pathway. Because female receptive behavior in primates is not fully dependent on estrogen, the question arises as to whether the capacity of estrogen-induced sprouting is preserved in primates. The effect of estrogen was studied on the NRA-lumbosacral projection with the use of wheat germ agglutinin conjugated to horseradish peroxidase as a tracer in six adult ovariectomized rhesus monkeys with or without estrogen priming (three controls and three treated with 20 microg/day of estradiol benzoate subcutaneously for 14 days). Light microscopy showed that the density of arborizing labeled NRA axons in the lumbosacral cord was greater in estrogen-treated than in control animals. Ultrastructurally, labeled NRA terminal profiles were quantified in motoneuron pools that supply muscles of the abdominal wall, axial, and pelvic floor. After estrogen treatment, the average number of labeled terminal profiles per area of the abdominal wall, axial, and pelvic floor motoneuron pool increased 1.5-, 3.3-, and 2.8-fold, respectively. In the estrogen-treated cases, 8.9% of labeled terminal profiles showed characteristics of growth cones. In controls, such profiles were rarely observed. The results showed that estrogen induces axonal sprouting in a brainstem-spinal pathway in the adult female rhesus monkey. These findings supported the concept that the NRA-lumbosacral pathway may be involved in sexual behavior. Moreover, they demonstrated that a long descending brainstem-spinal tract in adult nonhuman primates retains the capacity for axonal sprouting.