bFGF缓释微囊颗粒诱导角膜新生血管形成治疗兔大泡性角膜病变

目的:观察bFGF诱导角膜新生血管形成,对兔大泡性角膜病变的治疗作用。方法:采用0.5g/L苯扎溴铵溶液(BAKB)前房灌注复制兔大泡性角膜病变模型。然后将bFGF缓释微囊颗粒植入病变兔眼的角膜基质层间,诱导角膜新生血管生长,观察随角膜新生血管的形成,病变角膜水肿及大泡的改变。结果:在微囊颗粒植入后4wk病变角膜中央厚度2.61±0.25(CT),角膜水肿度评分0分,角膜新生血管评分0分,病变大泡呈吸收性改变。结论:大泡性角膜病变动物模型角膜基质层间植入bFGF缓释微囊颗粒,能够较为迅速的诱导角膜新生血管形成。同时,伴随CNV的生长,病变角膜的临床症状逐渐缓解。因此,该实验方法可能为大泡性角膜病变的治疗提供了另一种思路和方法。

Experimental study of treatment of bullous keratopathy by corneal neovascularization induced by slow release of bFGF micropellets in rabbit

·AIM:To observe corneal neovascularization(CNV)induced by slow release of bFGF micropellets for the treatment of bullous keratopathy.·METHODS:The model of bullous keratopathy was built by perfusing anterior chamber with 0.5g/L benzalkonium bromide solution.And then implant bFGF slow release micropellets to cornea propria of lagophthalmos with affection in order to induce CNV.Observe the degree of corneal edema and corneal bullous.·RESULTS:Four weeks after implanting bFGF slow release micropellets to cornea propria,the central corneal thickness of the cornea was 2.61±0.25(CT),corneal edema score was 0,and CNV score was 0.The bubble presented Absorbability changes.·CONCLUSION:The implantation of bFGF slow release micropellets to cornea propria in rabbit with bullous keratopathy can rapidly induce CNV.Moreover,the clinical symptoms of pathological cornea relieve gradually following with the growth of CNV.Therefore,the experimental method may provide another kind of idea and method for the treatment of bullous keratopathy.·