联合应用hIL-2和hGM-CSF基因瘤苗抗肿瘤作用的研究

目的：探讨联合应用人白细胞介素2（hIL-2)和人粒细胞巨呼细胞刺激因子（hGM-CSF)基因瘤苗抗肿瘤作用。方法：经逆转录病毒载体将hIL-2基因及hGM-CSF基因导入大鼠Walker瘤细胞WRC256中，制备瘤苗接种荷瘤大鼠。结果：经PCR－Southerm Blot检测证实hIL-2基因及hGM-CSF基因已成功导入WRC256细胞基因组中，经生物学活性检测证实转基因细胞具有分泌hIL-2及hGM-CSF活性。转基因瘤苗经60Gy X线灭活，接种荷瘤Walker瘤模型，两种瘤苗确可诱导有效抗肿瘤反应，但与单独应用一种瘤苗相比较，联合应用无相加或协同作用。结论：两种瘤苗可诱导有效抗肿瘤反应，但联合应用无相加或协同作用。

The Anti-tumour Investigations on the Tumour Vaccines Engineered by hIL-2 and hGM-CSF Genes

Objective: To investigate the Anti tumour effect of the tumour vaccines engineered by human interleukin 2(hIL 2) and human granulocyte macrophage colony stimulating factor(hGM CSF) genes. Methods: The hIL 2 and hGM CSF genes were transferred into the Walker tumour cell vaccinesbby virtue of retrovirus. The gene transferred vaccines were inoculated into the Walker tumour model.Results:Gene transfers were successfully confirmed by PCR Southern Blot. The biological activities of hIL 2 and hGM CSF by the gene transferred vaccine cells were showed with MTT assay. After in Vitro irradiation with 60Gy X ray, the gene transferred vaccines were inoculated into the Walker tumour model. Conclusion:The effective anti tumour response was induced by the vaccines. The plus or coordinate anti tumour effect by synchronous inoculation with two kinds of the vaccines was not observed.