新型表位基因疫苗的构建及对小鼠黑色素瘤的影响

目的：构建以MAGE-1（161—169）为表位的癌症基因疫苗并检测其抗小鼠黑色素瘤效果。方法：构建基因疫苗pcDNA—HSP70-MAGE—l（161-169），并免疫C57BL／6小鼠。最后一次免疫后第2周，接种小鼠黑色素瘤细胞。于肿瘤细胞接种后14d，处死全部动物，称量肿瘤的重量。采用ELISA法对小鼠血清中抗MAGE-1-IgG类抗体及小鼠原代脾淋巴细胞IFN-7释放水平进行检测。结果：pcDNA-HSP70-MAGE-1（161—169）表位基因疫苗能够成功地诱发机体产生抗MAGE-1的特异性抗体，并能在体内起到抗小鼠黑色素瘤作用，抑瘤率为40．7％。同时还能提高约3．05倍的小鼠原代脾淋巴细胞IFN-7释放量。结论：pcDNA-HSP70-MAGE-1（161-169）有望成为有效的抗小鼠黑色素瘤基因疫苗。

Suppressing effect of a novel epitopes DNA vaccine on mouse melanoma growth

Construction of MAGE-1_((161_169)) DNA vaccine and evaluated its anti-melanoma effects in mouse. METHODS: A DNA vaccine pcDNA3. 1-HSP70-MAGE-1_((161_169)) has been constructed and used to immunize mice 3 times at 2-weekly intervals. Two weeks after the last immunization, tumor challenge ex-periments were performed by using B16F10 melanoma cell. After 14 d of challenge experiments, all mice were sacrificed and tumors were weighted. The specific anti-MAGE-1 IgG antibodies and the IFN-γ released by mouse primary splenocytes were detected by ELISA methods. RESULTS: The specific anti-MAGE-1 anti-bodies were detected in the serum of the mice immu-nized with pcDNA3. 1-HSP70-MAGE-1_((161-169))DNA vaccine. It showed that B16F10 melanoma growth in mice of DNA vaccine group was obviously suppressed compared with that in saline control group , with tumor inhibitory rate of 40.7%. The IFN-γ released by mouse primary splenocytes in mice of HSP70-MAGE-1_((161-169)) DNA vaccine group was 3.05 times of that in saline control group. CONCLUSION: pcDNA3. 1-HSP70-MAGE-1_((161-169)) may be an effective DNA vac-cine for the treatment of MAGE-1 dependent tumors.