绞股蓝总苷对免疫功能低下小鼠模型特异性免疫功能的影响

目的:用绞股蓝总苷作为免疫增强剂治疗免疫低下小鼠模型,观察特异性免疫功能的变化。探讨绞股蓝总苷免疫增强的药理作用,观察剂量效果关系。方法:小鼠随机分为6组,模型组皮下注射环磷酰胺,制造免疫低下模型,与各对照组和药物高、中、低剂量组分别作血清溶血素实验和小鼠迟发型变态反应,观察各组特异性免疫功能变化。结果:模型小鼠的体液免疫功能和细胞免疫功能明显下降,而阳性药和高、中剂量绞股蓝均可明显地增强该免疫功能,绞股蓝总苷的作用强度呈剂量依赖性,高剂量组的作用略强于阳性药。结论:以较低剂量环磷酰胺成功造成小鼠免疫低下模型。绞股蓝总苷能非常明显地增强特异性免疫功能,作用呈剂量依赖性。最高剂量推荐200～400mg/kg。

Effects of Gypenosides to the Immune Deficit Mouse''''s Specific Immunological Function

Objective:The Gypenosides was used as an immune enhancer to cure the immune deficit mouse and observe specific immunological function by test and delayed hypersensitivity reaction test.The pharmacological funtion of immune enhancer of Gypenosides was evaluated and the dose-effect relationship was discussed.Methods:All of the mice were administrated with CTX and then were divided into six groups:control goup,high dose group,middle group,lowerdose group.Respectively,the hemolysin test and delayed hypersensitivity reaction test was performed in each group and the variation of specific immune function was observed.Results:The test showed that the index of hemolysin test and delayed hypersensitivity reaction test in control group was markedly decreased while the index of hemolysin test high and middle group was increased.The effect of Gypenosides was dose-dependent on the specific immune.Conclusion:The immune deficit model was established successfully by the administration of CTX.Gypenosides was showed markedly to increase the specific immune function and the effectwas dose dependent.The highest recommendation dose was between 200~400mg/kg.