IL-35通过诱导T细胞分化促进肺癌肿瘤进展

目的 探究肺癌中IL-35对T细胞表型及对癌症进展的影响.方法 建立小鼠肺癌移植瘤模型,施用IL-35中和抗体,监测肿瘤生长情况.并通过检测肿瘤中T细胞的浸润情况及其表型和细胞因子分泌情况,进一步探究对抗肿瘤免疫反应的影响.最后通过对肿瘤浸润T细胞的体外刺激实验,检测浸润T细胞的抗肿瘤活性.结果 瘤体积及瘤重的检测结果...

IL-35 promots tumor progression by inducing T cell differentiation in lung cancer

This study was designed to investigate the effect of IL-35 on T cell phenotype and cancer progression in lung cancer.The tumor model of lung cancer was established in mice,and the tumor growth was monitored after administration of IL-35 neutralizing antibody.The anti-tumor immune response was further explored by detecting the infiltration of T cells and cytokines production in the tumor.Finally,the anti-tumor activity of infiltrating T cells was detected by activation experiment in vitro.The detection results of tumor volume and tumor weight showed that IL-35 could inhibit the growth of lung tumor(P<0.05).At the same time,neutralizing IL-35 could also lead to less regulatory T cell infiltration(P<0.01),an increase in the proportion of cytotoxic T cells,decrease in the expression of inhibitory receptors on T cells(P<0.001)and increase in the production of IFN-γand TNF-α(P<0.01).In addition,the in vitro activation ability of tumor infiltrating T cells neutralized by IL-35 was also significantly enhanced(P<0.001).In conclusion,the neutralization of IL-35 can inhibit the growth of lung tumor,which is partly due to the decrease of tumor infiltrating regulatory T cells and the reversal of T cell exhaustion,thus enhancing the anti-tumor immune response.