络合萃取-反萃取制备甘草超滤液中芹糖甘草苷的工艺研究

目的 以芹糖甘草苷的萃取率、反萃取率为评价指标,建立一种甘草超滤液中芹糖甘草苷的分离纯化方法。方法 以芹糖甘草苷的萃取率为指标,通过单因素实验和U5(53)均匀设计筛选最佳的络合萃取剂组成、配比及萃取时间;以芹糖甘草苷的反萃取率为指标,筛选最佳的反萃取剂种类、质量分数及反萃取时间。结果 最佳络合萃取条件为13%三烷基氧膦（TRPO）+87%磺化煤油（TBP）萃取30min,芹糖甘草苷萃取率达85.69%;最佳反萃取条件为0.05%Na OH水溶液反萃取30 min,芹糖甘草苷反萃取率达88.35%。结论 优选所得的络合萃取-反萃取工艺条件可高效分离甘草超滤液中的芹糖甘草苷,可为芹糖甘草苷的制备提供一种新的技术。

Study on preparation technology of liquiritin apioside from licorice ultrafiltrate by complexation extraction and back extraction

Objective The extraction and reverse extraction rates of liquiritin apioside were used as evaluation indexes to establish a method for separation and purification of liquiritin apioside from licorice ultrafiltrate. Methods The extraction rate of liquiritin apioside was used as the index, based on the single factor experiment and U₅(5³) uniform design to determine the best composition and concentration of complexing extractant and extracion time. Taking the reverse extraction rate of liquiritin apioside as the index, the species and concentration of the reverse extraction and reverse extracion time were optimized. Results It was found that the best complexing extractant agent of liquiritin apioside was 13% TRPO + 87% sulfonated kerosene, extraction for 30 min, and extraction rate of liquiritin apioside reached 85.69%. The best reverse extraction agent was 0.05% NaOH aqueous solution, reverse extraction 30 minutes, and reverse extraction rate of liquiritin apioside reached 88.35%. Conclusion The optimized complexation extraction and reverse extraction has strong extraction capacity and high selectivity for liquiritin apioside in licorice ultrafiltrate, which can be used as a new process for the preparation of liquiritin apioside.