白细胞介素-11在小鼠脑缺血再灌注损伤中的保护作用及其机制

目的 探讨白细胞介素-11(interleukin-11,IL-11)在小鼠脑缺血再灌注损伤中的保护作用及机制研究.方法 选择SPF级C57BL/6雄性小鼠60只,随机分成为对照组、模型组和IL-11组,每组各20只,通过线栓法构建脑缺血再灌注损伤.应用TTC染色检测脑组织梗死面积,采用Longa评分法评估各组小鼠的神...

Protective effect of IL-11 against cerebral ischemia-reperfusion injury and its mechanism in mice

To investigate the protective effects of interleukin-11(IL-11)against cerebral ischemia reperfusion injury and the mechanism in mice,total of 60 male C57BL/6J mice were recruited and randomly divided into control group,model group and IL-11 group,with 20 mice in each group.The middle cerebral artery occlusion models were established.TTC staining was used to observe the area of cerebral infarction;Longa grade method was used to evaluate the neurobehavioral characteristics of mice,and the water content of brain tissue was measured.The expression levels of iNOS,IL-1β,CD206 and Aginase-1 in brain tissue were detected by Western blotting and the expression levels of IL-1,IL-6,IL-17 and TNF-αwere detected by ELISA.Immunofluorescence double staining was used to detect the effect of IL-11 on the differentiation of microglia.Compared with the control group,the area of cerebral infarction,Longa grade and the water content of brain tissue were significantly increased in model group.Meanwhile,those parameters were significantly decreased in IL-11 group.Compared with the control group,the number of M1 microglia in the model group was significantly increased;compared with the model group,the number of M1 microglia in the IL-11 group was decreased,and the number of M2 microglia was increased.Compared with the control group,the expression of type M1 microglia markers iNOS and IL-1βwere increased in the model group;compared with the model group,the expression of type M1 microglia markers iNOS and IL-1βwere decreased,while the expression of type M2 microglia markers CD206 and Aginase-1 were increased in the IL-11 group.Compared with the sham group,the levels of IL-1,IL-6,and TNF-αwere significantly increased,but IL-10 was significantly decreased in model group.Compared with the model group,the levels of IL-1,IL-6,and TNF-αwere significantly decreased,but IL-10 was significantly increased in IL-11 group.In conclusion,IL-11 promotes the transformation of microglia from pro-inflammatory phenotype M1 to anti-inflammatory phenotype M2,thus reduce brain inflammation and play a protective role in cerebral ischemia.