脊髓重建管对T8损伤大鼠后肢运动终板的调节与维持

目的观察大鼠脊髓损伤后及脊髓重建管诱导脊髓再生修复过程中后肢骨骼肌运动终板形态结构与不同类型肌纤维终板区乙酰胆碱酯酶(AChE)的变化情况。方法43只成年Wistar大鼠随机分为脊髓T₈单纯损伤组（Cx组）、脊髓T₈损伤+脊髓重建管移植修复组（CxTp组）和正常对照组（Co组），术后2周、4周、3个月、6个月、12个月经灌注固定后取出大鼠双下肢腓肠肌、比目鱼肌和趾长伸肌，分别用氯化金染色（压片法）观察运动终板结构形态和Karnovsky-Roots直接法染色检测AChE的变化。结果Cx组和CxTp组大鼠术后运动终板结构形态与AChE活性均发生改变。Cx组终板自术后3个月起发生明显的退行性改变，AChE活性亦呈线性下降；CxTp组终板结构与形态相对稳定，没有退变迹象，术后3个月起AChE 活性显著高于同时间点Cx组。结论大鼠脊髓损伤后，运动终板发生进行性退变，脊髓重建管移植修复可以阻止终板的退行性变，使其向着利于神经康复的方向发生着AChE的复活与结构形态的可塑性变化。

Adjustment and Maintenance of Spinal Cord Reconstruction Tubes on Hindlimb Motor End-plates about Spinal Cord Injury at T_8 in Rats

Objective To observe the hindlimb motor end-plate morphology and activity of acetyl cholinesterase (AChE) in different types of muscle fiber end-plate areas after spinal cord injury and spinal cord reconstruction tubes inducing spinal cord regeneration.Methods 43 adult Wistar rats were randomly divided into spinal cord transected group at T8 (Cx group),spinal cord transected with transplantation of reconstruction tubes group (CxTp group) and control group (Co group).2 weeks,4 weeks,3 months,6 months and 12 ...