Baicalin protects sertoli cells from heat stress-induced apoptosis via activation of the Fas/FasL pathway and Hsp72 expression |
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| Affiliation: | 1. Departamento de Ciência Animal, PPG Zootecnia, NESPRO, Universidade Federal do Rio Grande do Sul (UFRGS), 91540-000, Porto Alegre, RS, Brazil;2. Laboratório de Biotecnologia, Universidade do Vale do Taquari- Univates, 95914-014, Lajeado, RS, Brazil;3. Centro de Pesquisas em Biologia Molecular e Funcional (CPBMF), Instituto Nacional de Ciência e Tecnologia em Tuberculose (INCT-TB), Av. Ipiranga 6681 – Prédio 92A TecnoPuc (PUC), 90619-900, Porto Alegre, RS, Brazil;4. Departamento de Produção Animal, Universidade Norte do Paraná (UNOPAR), 86702-670, Arapongas, PR, Brazil;1. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China;2. University of the Chinese Academy of Sciences, Beijing 100101, PR China;3. College of Agriculture, Ningxia University, Yinchuan 750021, PR China;4. George Whipple Laboratory for Cancer Research, Departments of Pathology, The Wilmot Cancer Center, University of Rochester Medical Center, Rochester, NY 14623, USA;5. The Mary M. Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, NY 10065, USA;6. Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China;1. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China;2. College of Animal Science and Technology, Hebei Agricultural University, Baoding, 071000, China;3. National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China;1. Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada;2. Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;1. College of Animal Science, Yangtze University, Jingzhou, 434025, Hubei, China;2. College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China;3. College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, Anhui, China |
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| Abstract: | Certain Chinese herbal medicines have antipyretic effects in both animal and human clinical practice. However, no report indicates their antipyretic effects on heat-stressed cells. The present study aimed to identify the protective effects of baicalin on the apoptosis of primary cultured bovine sertoli cells (SCs) subjected to heat stress (HS). The results demonstrated that HS induced apoptosis in the SCs exposed to 43 °C for 1 h as Fas/FasL was activated and caspase-3 was cleaved, the cells apoptotic rate was decreased. Moreover, the mRNA and protein levels of Hsp72 increased, whereas the cells apoptotic rate and expression of Fas, FasL, caspases 8 and 3 decreased in the SCs pretreated with various concentrations (0.1, 1, 10, 20 μg/mL) of baicalin prior to HS. In conclusion, baicalin ameliorates heat stress-induced cell apoptosis via the modulation of the cell survival rate through Fas/FasL pathway activation and the upregulation of Hsp72 expression in bovine SCs. |
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| Keywords: | Bovine sertoli cells Baicalin Heat stress Apoptosis Hsp72 |
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