中国新疆维吾尔族Rh血型系统RHCE基因分型的研究

本研究目的旨在探讨新疆维吾尔族RHCE基因分型特点。根据文献设计RHCE基因检测的引物,采用序列特异性引物-聚合酶链反应(SSP-PCR)检测非血缘关系RhD阴性样本维吾尔族89例、汉族233例及RhD阳性样本汉族109例。结果表明,维吾尔族及汉族无论RhD阴性或RhD阳性全部样本中RHE/e基因分型结果与血清学检测结果一致;89例维吾尔族RhD阴性样本RHC/c等位基因分型结果与其血清学检测结果一致,而233例汉族RhD阴性及109例汉族RhD阳性样本中RHC/c基因错误定型率为5.05%。结论:本研究方法适合用于维吾尔族RHE/e基因分型,在本研究中用于维吾尔族RHC/c基因分型中未发现有错误,但今后仍需进一步验证。

Study on RHCE Genotyping of Rh Blood Group in Uygur Nationality of Xinjiang in China

This study was aimed to investigate the characteristics of RHCE genotyping of Xinjiang Uygur nationality population in China.Primers for detecting RHCE genes were designed according to the references,89 Uygur nationality RhD-negative samples,233 Han nationality RhD-negative samples and 109 Han nationality RhD-positive samples were detected by sequence-specific primer-polymerase chain reaction（SSP-PCR） for RHCE genotyping.All above-mentioned samples were unrelated.The results indicated that RHE/e genotyping results were consistent with the serological test results in the samples of Uygur and Han nationality,regardless of the RhD-negative samples or the RhD-positive samples.The RHC/c genotyping results from 89 RhD-negative samples of Uygur nationality were consistent with serological test results.However,total error of RHC/c genotyping from 233 RhD-negative and 109 RhD-positive samples of Han nationality was 5.05%.In conclusion,this method of RHCE genotyping is suitable for the analysis of the RHE/e genotyping of Uygur nationality,no erroneous RHC/c genotyping of Uygur nationality was found in this study,but this method needs to be further studied.