戒毒康酊剂中氢溴酸东莨菪碱及总生物碱的含量测定

目的测定中药戒毒康酊剂中氢溴酸东莨菪碱及总生物碱的含量。方法采用DiamonsilC18柱（250mm×4．6mm，5μm）分离测定氢溴酸东莨著碱，流动相：以甲醇-0．02mol·L^-1醋酸钠溶液含O．02％三乙胺和0．3％四氢呋喃．用冰醋酸调pH到6．2）梯度洗脱；流速lmL·min^-1；检测波长：206nm。采用酸性染料比色法测定戒毒康中的总生物碱，O．2％溴百里香酚兰溶液显色，420nm处测得吸光度。结果氢溴酸东莨菪碱在1．0～5．0μg内呈良好的线性关系（r=0．9996）；平均回收率为98．8％，RSD为2．29％（n=6）。总生物碱在0．04～0．2mg内呈良好的线性关系（r=0．9998）；平均回收率为97．6％．RSD为2．06％（n=6）。结论衣方法简便、快速、准确、专属性强，可作为戒毒康酊剂的检验方法．

Determination of Scopolamine Hydrobromide and Total Alkaloid in Jiedukang Tincture

OBJECTIVE To establish an HPLC method for determination of contents of scopolamine hydrobromide and total alkaloid in Jiedukang tincture.METHODS A Diamonsil C18 column(250 mm×4.6 mm,5 μm) was used to isolate and determine scopolamine hydrobromide.The mobile phase was methanol-0.02 mol·L 1 NaAc solution(with 0.02% triethylamine and 0.3% tetrahydrofuran,adjusted to pH 6.2 by HAc).The flow rate was 1 mL·min 1.The detection wavelength was set at 206 nm.Total alkaloids in Jiedukang tincture were determined with acid dye colorimetry.0.2% Bromothymol blue was used as the dye reagent and the wavelength was set at 420 nm.RESULTS The linear range of scopolamine hydrobromide was 1.0 5.0 μg with a good correlation(r=0.999 6).The average recoveries was 98.8% with RSD of 2.29%(n=6).The total alkaloids showed good linearity within the range of 0.04 0.2 mg(r=0.999 8),the average recoveries was 97.6% with RSD of 2.06%(n=6).CONCLUSION The method established is sensitive,accurate and can be used for determination of scopolamine hydrobromide in Jiedukang tincture.