| 抗人肺癌mAbLC-1的人-鼠嵌合Fab片段基因表达质粒的构建与表达 |
| |
| 引用本文： | 陈良,谢荷煌,王珏,范赵娟,林心楷,陈松华,刘伟,葛锡锐.抗人肺癌mAbLC-1的人-鼠嵌合Fab片段基因表达质粒的构建与表达[J].细胞与分子免疫学杂志,2000,16(1):063-066. |
| |
| 作者姓名： | 陈良 谢荷煌 王珏 范赵娟 林心楷 陈松华 刘伟 葛锡锐 |
| |
| 基金项目： | 世界实验室MCD 1B计划资助!No.1 IV，上海市科技发展基金!No.984419071 |
| |
| 摘 要： | 目的表达抗人肺癌单克隆抗体(mAb)LC 1的人 鼠嵌合基因工程抗体。方法将mAbLC 1的VL 和VH 基因插入质粒 pWS1 中 ,构建成表达mAbLC 1人 鼠嵌合Fab片段基因的质粒pLC 1 ,转化大肠杆菌TG 1并在其中表达。结果酶切鉴定表明 ,mAbLC 1的VL 和VH 基因插入方向正确 ,该载体能在大肠杆菌TG1中表达预期相对分子质量的蛋白 ,表达产物对mAb具有较强的竞争结合抗原的活性。结论成功地表达了具有较高亲和活性的人 鼠嵌合抗人肺癌的基因工程抗体。
|
| 关 键 词： | 肺癌 单克隆抗体 质粒 原核表达 |
| 修稿时间： | 1999－01－28 |
Construction
and expression of recombinant plasmid pLC-1 bearing human-mouse chimeric Fab fragment gene
of mAb LC-1 to human pulmonary carcinoma |
| |
| CHEN Liang,XIE He huang,WANG Jue,FAN Zhao juan,LIN Xin kai,CHEN Song hua,LIU Wei,GE Xi ruiInstitute of Cell Biology,Chinese Academy of Sciences,Shanghai ,China.Construction
and expression of recombinant plasmid pLC-1 bearing human-mouse chimeric Fab fragment gene
of mAb LC-1 to human pulmonary carcinoma[J].Journal of Cellular and Molecular Immunology,2000,16(1):063-066. |
| |
| Authors: | CHEN Liang XIE He huang WANG Jue FAN Zhao juan LIN Xin kai CHEN Song hua LIU Wei GE Xi ruiInstitute of Cell Biology Chinese Academy of Sciences Shanghai China |
| |
| Institution: | CHEN Liang,XIE He huang,WANG Jue,FAN Zhao juan,LIN Xin kai,CHEN Song hua,LIU Wei,GE Xi ruiInstitute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China |
| |
| Abstract: | Aim To express human mouse chimeric genetic engineering antibody of monoclonal antibody(mAb)LC 1 to human pulmonary carcinoma. Methods VL and VH genes of the mAb LC 1 were cloned into plasmid pSW1 and then plasmid pLC 1 expressing human mouse chimeric Fab fragment gene of the mAb LC 1 was constructed. The plasmid pLC 1 was transformed into E.coli TG 1 and expressed in it. Results Restriction enzyme digestion showed that the VL and VH genes of the mAb LC 1 were inserted into plasmid pSW1 in correct direction. Expression products of plasmid pSW1 in E.coli TG 1 could compete against mAb LC 1 in binding to antigen. Conclusion Human mouse chimeric genetic engineering antibody to human pulmonary carcinoma is successfully expressed, with higher affinity activity. |
| |
| Keywords: | pulmonary carcinoma monoclonal antibody plasmid prokaryotic expression |
|
|
