| 乙型肝炎病毒基因组准种与变异特点的研究 |
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| 引用本文： | 董菁,李进,施双双,皇甫竞坤,成军,王勤环,洪源,李莉. 乙型肝炎病毒基因组准种与变异特点的研究[J]. 解放军医学杂志, 2002, 27(2): 116-118 |
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| 作者姓名： | 董菁 李进 施双双 皇甫竞坤 成军 王勤环 洪源 李莉 |
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| 作者单位： | 100039,北京,解放军第302医院传染病研究所基因治疗研究中心;北京大学第一医院感染疾病科 |
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| 摘 要： | 探讨乙型肝炎病毒（HBV）基因组异质性及其生物学意义。以已知HBV基因序列为依据，设计特异性多聚酶链反应（PCR）引物，自2例慢性HBV感染患者外周血血清中扩增HBV基因组序列，克隆入pGEM Teasy质粒，随机挑选克隆进行DNA测序并加以比较。测序结果发现，来源不同的5株HBV全基因组核苷酸序列的一致率为93．6％，不同克隆的4个开放读码框架长度有明显区别，氨基酸序列中存有移框、缺失、替换等多种变异类型。说明来源于同一患者的HBV基因组序列之间存在有较明显的变异现象，在患者体内构成了准种群。
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| 关 键 词： | 肝炎病毒 乙型 基因组 变异(遗传学) 准种 |
| 修稿时间： | 2001-12-20 |
THE STUDY ON QUASISPECIES OF HEPATITIS B VIRUS (HBV):HBV GENOME AS AN EXAMPLE |
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| Dong Jing,Li Jin,Shi Shuangshuang et al . Gene Therapy Research Center. THE STUDY ON QUASISPECIES OF HEPATITIS B VIRUS (HBV):HBV GENOME AS AN EXAMPLE[J]. Medical Journal of Chinese People's Liberation Army, 2002, 27(2): 116-118 |
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| Authors: | Dong Jing Li Jin Shi Shuangshuang et al . Gene Therapy Research Center |
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| Affiliation: | Dong Jing,Li Jin,Shi Shuangshuang et al . Gene Therapy Research Center,Institute of Infectious Diseases,the 302 Hospital of PLA,Beijing 100039 |
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| Abstract: | To investigate the HBV quasispecies groups in the patients with chronic HBV infection. A set of specific primers was synthesized according to HBV DNA sequence, the whole genome was amplified by PCR method from the serum of 2 patients with chronic HBV infection, and then the PCR products were subcloned into pGEM Teasy vectors. Five clones were randomly selected to be sequenced. Sequence comparison showed that each sequence of selected clones was distinctly different, and the nucleic acid identity rate of these 5 clones was 93 6%. Substitution, deletion and frame shift were found in the amino acid sequences of 4 ORFs from the 5 clones. The sequencing comparison implies that there were HBV quasispecies groups in chronically infected patients. |
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| Keywords: | hepatitis B virus genome variation(genetics) quasispecies |
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