胸水肺癌细胞内质网的激光共聚焦显微镜研究

目的利用激光扫描共聚焦显微镜（LSCM）技术对胸水中肺癌细胞内质网、细胞核进行平面、立体、动态、荧光含量研究,为肺癌的诊断、鉴别诊断、细胞分化程度、来源和功能状态判断提供依据。方法将标本分为肺癌组及正常对照组,用荧光探针DioC6（3）标记内质网、DAPI标记细胞核,得到内质网断层扫描图像、荧光强度地形立体分布图及细胞核荧光扫描图,并定量测量内质网、细胞核的荧光强度值。结果肺癌组与正常对照组相比,内质网及细胞核空间立体分布均有明显差别,平均荧光值差异有统计学意义（P〈0.05）,细胞核荧光强度与核周长、核面积呈正相关。结论 LSCM技术对肺癌的诊断、鉴别诊断、细胞分化程度、来源和功能状态判断有一定的临床意义。

Observation of lung cancer cell in the hydrothorax by laser scanning confocal microscope

Objective To investigate the endoplasmic reticulum and nucleus of lung cancer cell by laser scanning confocal microscope（LSCM）to three-dimensionally,dynamically and quantitatively.Methods The subjects were divided into normal cells group and lung cancer cell group,endoplasmic reticulum were stained with fluorescent probe DioC6（3）and nucleus were stained with DAPI.Sectional scanograph and fluorescent intensity stereophotography were obtained and fluorescent values of cellular endoplasmic reticulum and nucleus were individually measured.Results Lung cancer cell compared with normal control group,endoplasmic reticulum and nucleus showed significant differences in the distribution of spatial three-dimensional（P0.05）,with an average fluorescence value was significant difference in fluorescence intensity of nuclei and nuclear perimeter,nuclear area positively correlated（P0.05）.Conclusion LSCM technique has clinical significance of certain for lung cancer diagnosis,differential diagnosis,cell differentiation,origin and function of the state.