实时荧光定量聚合酶链反应检测复发性阿弗他溃疡患者中人类疱疹病毒5型和8型的潜伏

目的采用实时荧光定量聚合酶链反应(PCR)检测轻型复发性阿弗他溃疡(RAU)患者4种样本中人类疱疹病毒5型(HHV-5)和8型(HHV-8)的潜伏情况。方法选择18例轻型RAU患者为试验组,同时选择健康者23名为对照组进行临床采样,样本包括唾液、病变区脱落细胞、病变区组织块及外周血。然后用实时荧光定量PCR检测4种样本中HHV-5和HHV-8的DNA。结果HHV-5仅在试验组患者病损区组织块样本中能检测到,而在对照组的组织块样本中没有检测到。试验组和对照组的血液和唾液样本中均有HHV-5阳性检出,但两组比较其差异无统计学意义(P>0.05);试验组和对照组的脱落细胞样本中均无HHV-5阳性检出。试验组和对照组的4种样本中HHV-8均检出阴性。结论HHV-5在RAU患者的溃疡区可能有潜伏。

Employed real-time fluorescent quantitative polymerized chain reaction to quantified human herpes viruses-5 and 8 in recurrent aphthous ulcer samples

Objective To test the DNT of human herpes virus-5 and 8(HHV-5 and 8) located in four kinds of samples obtained from minor recurrent aphthous ulcer(MiRAU) patients using the real-time fluorescent quantitative PCR. Methods Samples of the saliva, exfoliated cells, biopsy tissues and the blood of 18 MiRAU patients and 23 health persons were simultaneously collected in one patient or person by oral medicine specialists. They were exactly determined by the real-time fluorescent quantitative PCR. Results The HHV-5 positive rate in MiRAU biopsy samples had significant statistics difference with their health controls(P<0.05). However, the HHV-5 positive rate in MiRAU saliva and blood samples did not have significant test with their health controls(P>0.05). In another part, we did not find the DNA of HHV-5 in exfoliated cells of both groups and HHV-8 in either group. Conclusion It is supposed that HHV-5 may be concealed in immune cells and duplicated in oral small salary gland tissue cells on account of characters of recurrent occurrence and the heal postponement in MiRAU patients.